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PMID: 1371461 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of a constitutive form of calcium/calmodulin dependent protein kinase II leads to arrest of the cell cycle in G2.

The EMBO journal ·Vol. 11 ·No. 2 ·1992-02-00 ·Pages 507-17

Planas-Silva MD, Means AR

Abstract

Calcium/calmodulin dependent protein kinase II (CaMKII) is a multifunctional serine/threonine protein kinase. We have created a calcium/calmodulin independent form of this enzyme by truncation. Expression of this enzyme fragment in a rabbit reticulocyte lysate yields a constitutive enzyme with specific activity similar to the activated native enzyme. We have established mammalian cell lines that transiently express this constitutive enzyme using the glucocorticoid-inducible mouse mammary tumor virus long terminal repeat. The transient increase in kinase activity results in a complete cessation of cell cycle progression. This block develops as a consequence of a specific arrest of the cell cycle in G2. During the block, increases in histone H1 kinase activity present in p13 beads or anti-cdc2 immunoprecipitates are seen in parallel with the accumulation of cells at G2, arguing that the arrest is not due to a failure to activate cdc2 as a histone H1 kinase. These results suggest that other changes in serine/threonine protein phosphorylation besides those involved in activation of cdc2 as a histone H1 kinase may be necessary for proper G2-M transition.

MeSH Terms
Amino Acid Sequence Animals Blotting, Western Brain/enzymology CDC2 Protein Kinase/analysis,metabolism Calcium-Calmodulin-Dependent Protein Kinases Cell Cycle/drug effects,physiology Cell Line Chromosome Deletion Cyclins/analysis,metabolism Dexamethasone/pharmacology Fluorescent Antibody Technique Genetic Vectors Mammary Tumor Virus, Mouse/genetics Mice Molecular Sequence Data Plasmids Protamine Kinase/analysis,metabolism Protein Biosynthesis Protein Kinases/analysis,genetics,metabolism RNA/genetics,isolation & purification Repetitive Sequences, Nucleic Acid Transcription, Genetic/drug effects Transfection
Chemicals
Cyclins RNA Dexamethasone Protein Kinases Protamine Kinase Calcium-Calmodulin-Dependent Protein Kinases CDC2 Protein Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Planas-Silva M D
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
Means A R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-02-00
Pages
507-17
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556481
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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