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PMID: 1372388 Published · ppublish English Journal Article

A lymphoid cell-specific nuclear factor containing c-Rel-like proteins preferentially interacts with interleukin-6 kappa B-related motifs whose activities are repressed in lymphoid cells.

Molecular and cellular biology ·Vol. 12 ·No. 4 ·1992-04-00 ·Pages 1736-46

Nakayama K, Shimizu H, Mitomo K, Watanabe T, Okamoto S, Yamamoto K

Abstract

The proto-oncoprotein c-Rel is a member of the nuclear factor kappa B transcription factor family, which includes the p50 and p65 subunits of nuclear factor kappa B. We show here that c-Rel binds to kappa B sites as homodimers as well as heterodimers with p50. These homodimers and heterodimers show distinct DNA-binding specificities and affinities for various kappa B motifs. In particular, the c-Rel homodimer has a high affinity for interleukin-6 (IL-6) and beta interferon kappa B sites. In spite of its association with p50 in vitro, however, we found a lymphoid cell-specific nuclear factor in vivo that contains c-Rel but not p50 epitopes; this factor, termed IL-6 kappa B binding factor II, appears to contain the c-Rel homodimer and preferentially recognizes several IL-6 kappa B-related kappa B motifs. Although it has been previously shown that the IL-6 kappa B motif functions as a potent IL-1/tumor necrosis factor-responsive element in nonlymphoid cells, its activity was found to be repressed in lymphoid cells such as a Jurkat T-cell line. We also present evidence that IL-6 kappa B binding factor II functions as a repressor specific for IL-6 kappa B-related kappa B motifs in lymphoid cells.

MeSH Terms
Animals Base Sequence Cells, Cultured Cytokines/pharmacology Epitopes Gene Expression Regulation Humans Interleukin-6/genetics Macromolecular Substances Models, Genetic Molecular Sequence Data Promoter Regions, Genetic/genetics Proto-Oncogene Proteins/metabolism Proto-Oncogene Proteins c-rel Sequence Homology, Nucleic Acid T-Lymphocytes/drug effects,metabolism Tissue Distribution Transcription Factors/metabolism
Chemicals
Cytokines Epitopes Interleukin-6 Macromolecular Substances Proto-Oncogene Proteins Proto-Oncogene Proteins c-rel Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Nakayama K
Department of Molecular Pathology, Kanazawa University, Ishikawa, Japan.
Shimizu H
Mitomo K
Watanabe T
Okamoto S
Yamamoto K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.4.1736-1746.1992
Published
1992-04-00
Pages
1736-46
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369617
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.12.4.1736-1746.1992 PMC Full Text View journal details
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