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PMID: 2204816 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The mouse c-rel protein has an N-terminal regulatory domain and a C-terminal transcriptional transactivation domain.

Molecular and cellular biology ·Vol. 10 ·No. 10 ·1990-10-00 ·Pages 5473-85

Bull P, Morley KL, Hoekstra MF, Hunter T, Verma IM

Abstract

We have shown that the murine c-rel protein can act as a transcriptional transactivator in both yeast and mammalian cells. Fusion proteins generated by linking rel sequences to the DNA-binding domain of the yeast transcriptional activator GAL4 activate transcription from a reporter gene linked in cis to a GAL4 binding site. The full-length mouse c-rel protein (588 amino acids long) is a poor transactivator; however, the C-terminal portion of the protein between amino acid residues 403 to 568 is a potent transcriptional transactivator. Deletion of the N-terminal half of the c-rel protein augments its transactivation function. We propose that c-rel protein has an N-terminal regulatory domain and a C-terminal transactivation domain which together modulate its function as a transcriptional transactivator.

MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA Mutational Analysis DNA-Binding Proteins/physiology Gene Expression Regulation Mice Molecular Sequence Data NF-kappa B Plasmids Polymerase Chain Reaction Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-rel Proto-Oncogenes Recombinant Fusion Proteins/physiology Regulatory Sequences, Nucleic Acid Restriction Mapping Transcription Factors/physiology Transcription, Genetic
Chemicals
DNA-Binding Proteins NF-kappa B Proto-Oncogene Proteins Proto-Oncogene Proteins c-rel Recombinant Fusion Proteins Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bull P
Molecular Biology and Virology Laboratory, Salk Institute, San Diego, California 92138.
Morley K L
Hoekstra M F
Hunter T
Verma I M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.10.5473-5485.1990
Published
1990-10-00
Pages
5473-85
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361256
Subset
IM
Grants
NCI NIH HHS · CA14195 · United States
NCI NIH HHS · CA44360 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.10.5473-5485.1990 PMC Full Text View journal details
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