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PMID: 1379177 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Co-ordinate expression of the two threonyl-tRNA synthetase genes in Bacillus subtilis: control by transcriptional antitermination involving a conserved regulatory sequence.

The EMBO journal ·Vol. 11 ·No. 8 ·1992-08-00 ·Pages 3117-27

Putzer H, Gendron N, Grunberg-Manago M

Abstract

In Bacillus subtilis, two genes, thrS and thrZ, encode distinct threonyl-tRNA synthetase enzymes. Normally, only the thrS gene is expressed. Here we show that either gene, thrS or thrZ, is sufficient for normal cell growth and sporulation. Reducing the intracellular ThrS protein concentration induces thrZ expression in a dose-compensatory manner. Starvation for threonine simultaneously induces thrZ and stimulates thrS expression. The 5'-leader sequences of thrS and thrZ contain, respectively, one and three transcription terminators preceded by a conserved sequence. We show that this sequence is essential for the regulation of thrS via a transcriptional antitermination mechanism. We propose that both genes, thrS and thrZ, are regulated by the same mechanism such that the additional regulatory domains present before thrZ account for its non-expression. In contrast to Escherichia coli, structurally similar regulatory domains, i.e. the consensus sequence preceding a terminator structure, are found in the leader regions of most aminoacyl-tRNA synthetase genes of Gram-positive bacteria. This suggests that they are regulated by a common mechanism.

Related Genes
lacZ thrS thrZ
MeSH Terms
Bacillus subtilis/enzymology,genetics Base Sequence Chromosomes, Bacterial DNA, Bacterial/genetics,metabolism Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic Genes, Bacterial Genes, Lethal Genes, Regulator Genotype Kinetics Molecular Sequence Data Multigene Family Open Reading Frames Plasmids RNA, Bacterial/genetics,isolation & purification Recombinant Fusion Proteins/isolation & purification,metabolism Suppression, Genetic Threonine-tRNA Ligase/genetics,isolation & purification,metabolism Transcription, Genetic beta-Galactosidase/genetics,metabolism
Chemicals
DNA, Bacterial RNA, Bacterial Recombinant Fusion Proteins beta-Galactosidase Threonine-tRNA Ligase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Putzer H
CNRS, URA 1139, Institut de Biologie Physico-Chimique, Paris, France.
Gendron N
Grunberg-Manago M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-08-00
Pages
3117-27
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556796
Subset
IM
Databases
GENBANK
X04963, X66121, X66358, X66359, X66360, X66361, X66362, X66363, X66364, X66365
External Links
PubMed source PMC Full Text View journal details
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