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PMID: 2183178 Published · ppublish English Comparative Study Journal Article

Homology of lysS and lysU, the two Escherichia coli genes encoding distinct lysyl-tRNA synthetase species.

Nucleic acids research ·Vol. 18 ·No. 2 ·1990-01-25 ·Pages 305-12

Lévêque F, Plateau P, Dessen P, Blanquet S

Abstract

In Escherichia coli, two distinct lysyl-tRNA synthetase species are encoded by two genes: the constitutive lysS gene and the thermoinducible lysU gene. These two genes have been isolated and sequenced. Their nucleotide and deduced amino acid sequences show 79% and 88% identity, respectively. Codon usage analysis indicates the lysS product being more efficiently translated than the lysU one. In addition, the lysS sequence exactly coincides with the sequence of herC, a gene which is part of the prfB-herC operon. In contrast to the recent proposal of Gampel and Tzagoloff (1989, Proc. Natl. Acad. Sci. USA 86, 6023-6027), the lysU sequence is distinct from the open reading frame located adjacent to frdA, although large homologies are shared by these two genes.

MeSH Terms
Amino Acid Sequence Amino Acyl-tRNA Synthetases/genetics Base Sequence Cloning, Molecular DNA Probes DNA, Bacterial/genetics,isolation & purification DNA, Recombinant/metabolism Escherichia coli/enzymology,genetics Gene Library Genes, Bacterial Isoenzymes/genetics,isolation & purification Lysine-tRNA Ligase/genetics,isolation & purification Molecular Sequence Data Plasmids Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
DNA Probes DNA, Bacterial DNA, Recombinant Isoenzymes Amino Acyl-tRNA Synthetases Lysine-tRNA Ligase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lévêque F
Laboratoire de Biochimie de l'Ecole Polytechnique, Unité associée 240 du Centre National de la Recherche Scientifique, Palaiseau, France.
Plateau P
Dessen P
Blanquet S
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.2.305
Published
1990-01-25
Pages
305-12
Language
English
Region
England
NLM ID
0411011
PMCID
PMC330268
Subset
IM
Databases
GENBANK
X16542
External Links
PubMed source DOI: 10.1093/nar/18.2.305 PMC Full Text View journal details
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