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PMID: 3513164 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Evolution in biosynthetic pathways: two enzymes catalyzing consecutive steps in methionine biosynthesis originate from a common ancestor and possess a similar regulatory region.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 4 ·1986-02-00 ·Pages 867-71

Belfaiza J, Parsot C, Martel A, de la Tour CB, Margarita D, Cohen GN, Saint-Girons I

Abstract

The metC gene of Escherichia coli K-12 was cloned and the nucleotide sequence of the metC gene and its flanking regions was determined. The translation initiation codon was identified by sequencing the NH2-terminal part of beta-cystathionase, the MetC gene product. The metC gene (1185 nucleotides) encodes a protein having 395 amino acid residues. The 5' noncoding region was found to contain a "Met box" homologous to sequences suggestive of operator structures upstream from other methionine genes that are controlled by the product of the pleiotropic regulatory metJ gene. The deduced amino acid sequence of beta-cystathionase showed extensive homology with that of the MetB protein (cystathionine gamma-synthase) that catalyzes the preceding step in methionine biosynthesis. The homology strongly suggests that the structural genes for the MetB and MetC proteins evolved from a common ancestral gene.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Carbon-Oxygen Lyases Cloning, Molecular Escherichia coli/enzymology Genes Genes, Bacterial Lyases/genetics Phylogeny Recombinant Proteins/genetics Sequence Homology, Nucleic Acid
Chemicals
Bacterial Proteins Recombinant Proteins O-succinylhomoserine (thiol)-lyase Lyases Carbon-Oxygen Lyases cystathionine beta-lyase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Belfaiza J
Parsot C
Martel A
de la Tour C B
Margarita D
Cohen G N
Saint-Girons I
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.4.867
Published
1986-02-00
Pages
867-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC322971
Subset
IM
Databases
GENBANK
M12858
External Links
PubMed source DOI: 10.1073/pnas.83.4.867 PMC Full Text View journal details
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