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PMID: 6356036 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of metF, the E. coli structural gene for 5-10 methylene tetrahydrofolate reductase and of its control region.

Nucleic acids research ·Vol. 11 ·No. 19 ·1983-10-11 ·Pages 6723-32

Saint-Girons I, Duchange N, Zakin MM, Park I, Margarita D, Ferrara P, Cohen GN

Abstract

The nucleotide sequence of the E.coli metF gene (888 nucleotides), coding for 5-10 methylene tetrahydrofolate reductase, has been determined. The metF gene product was identified in maxicells and found to be a protein of subunit molecular weight 33,000, in agreement with the size of the coding region. The starting point for metF transcription was determined by S1 nuclease mapping. No structural evidence was found for an attenuation mechanism regulating the independent metF transcriptional unit. Comparison of the regulatory region preceding the metF structural gene with the 5' flanking region of the metBL operon shows some homology spanning 24 nucleotides. These homologous sequences could be operator structures belonging to the two transcriptional units, metF and metBL, and recognized by the same regulatory protein.

MeSH Terms
5,10-Methylenetetrahydrofolate Reductase (FADH2) Alcohol Oxidoreductases/genetics,isolation & purification Amino Acid Sequence Base Sequence Escherichia coli/enzymology,genetics Genes Genes, Bacterial Macromolecular Substances Molecular Weight Operon Transcription, Genetic
Chemicals
Macromolecular Substances Alcohol Oxidoreductases 5,10-Methylenetetrahydrofolate Reductase (FADH2)
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Saint-Girons I
Duchange N
Zakin M M
Park I
Margarita D
Ferrara P
Cohen G N
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21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.19.6723
Published
1983-10-11
Pages
6723-32
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326410
Subset
IM
Databases
GENBANK
V01502
External Links
PubMed source DOI: 10.1093/nar/11.19.6723 PMC Full Text View journal details
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