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PMID: 6219268 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Construction and physical mapping of plasmids containing the metJBLF gene cluster of E. coli K12.

Molecular & general genetics : MGG ·Vol. 187 ·No. 1 ·1982-00-00 ·Pages 101-6

Zakin MM, Greene RC, Dautry-Varsat A, Duchange N, Ferrara P, Py MC, Margarita D, Cohen GN

Abstract

In vitro recombination techniques were used to clone the E. coli metJBLF gene cluster in a plasmid vector. Several chimeric plasmids were obtained, analyzed by restriction mapping and characterized genetically. The combined results establish that the met gene cluster is contained on an approximately 5.6 kilobase segment of bacterial DNA with metL between metB and metF. The origin of metL was localized precisely by its DNA sequence and its transcription direction was established.

MeSH Terms
Bacteriophage lambda/genetics Base Sequence Cloning, Molecular DNA, Bacterial/genetics DNA, Recombinant DNA, Viral/genetics Escherichia coli/genetics Genes, Bacterial Plasmids
Chemicals
DNA, Bacterial DNA, Recombinant DNA, Viral
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Zakin M M
Greene R C
Dautry-Varsat A
Duchange N
Ferrara P
Py M C
Margarita D
Cohen G N
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30 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00384390
Published
1982-00-00
Pages
101-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
PHS HHS · 6M10317 · United States
External Links
PubMed source DOI: 10.1007/BF00384390
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