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PMID: 7003595 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the thrA gene of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 10 ·1980-10-00 ·Pages 5730-3

Katinka M, Cossart P, Sibilli L, Saint-Girons I, Chalvignac MA, Le Bras G, Cohen GN, Yaniv M

Abstract

The thrA gene of Escherichia coli codes for a single polypeptide chain having two enzymatic activities required for the biosynthesis of threonine, aspartokinase I and homoserine dehydrogenase I. This gene was cloned in a bacterial plasmid and its complete nucleotide sequence was established. It contains 2460 base pairs that encode for a polypeptide chain of 820 amino acids. The previously determined partial amino acid sequence of this protein is in good agreement with that predicted from the nucleotide sequence. The gene contains an internal sequence that resembles the structure of bacterial ribosome-binding sites, with an AUG preceded by four triplets, each of which can be converted to a nonsense codon by a single mutation. This suggests that the single polypeptide chain was formed by the fusion of two genes and that initiation of translation may occur inside the gene to give a protein fragment having only the homoserine dehydrogenase activity.

MeSH Terms

Aspartokinase Homoserine Dehydrogenase/genetics Cloning, Molecular Codon DNA, Bacterial/genetics Escherichia coli/genetics Genes Multienzyme Complexes/genetics Nucleic Acid Conformation Protein Conformation RNA, Messenger/genetics

Chemicals

Codon DNA, Bacterial Multienzyme Complexes RNA, Messenger Aspartokinase Homoserine Dehydrogenase

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Katinka M

Cossart P

Sibilli L

Saint-Girons I

Chalvignac M A

Le Bras G

Cohen G N

Yaniv M

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.77.10.5730

Published

1980-10-00

Pages

5730-3

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC350143

Subset

Databases

GENBANK

J01706, J01707, J01708, J01709, V00360, X00092

External Links

PubMed source DOI: 10.1073/pnas.77.10.5730 PMC Full Text View journal details

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Nucleotide sequence of the thrA gene of Escherichia coli.

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