Mapping of the constitutive lysyl-tRNA synthetase gene of Escherichia coli K-12.

Journal of bacteriology ·Vol. 169 ·No. 11 ·1987-11-00 ·Pages 5311-3

Emmerich RV, Hirshfield IN

Abstract

The constitutive lysyl-tRNA synthetase gene (lysS) was mapped at 62.1 min on the Escherichia coli chromosome by a combination of conjugation and transduction, with physical confirmation by two-dimensional gel electrophoresis. Revertant analysis suggests that the altered isoelectric point and the low amount of the mutant LysS protein may be due to a single mutational event.

MeSH Terms

Amino Acyl-tRNA Synthetases/genetics Chromosome Mapping Chromosomes, Bacterial/physiology Escherichia coli/enzymology,genetics Genes Genes, Bacterial Genotype Lysine-tRNA Ligase/genetics Phenotype

Chemicals

Amino Acyl-tRNA Synthetases Lysine-tRNA Ligase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Emmerich R V

Department of Biological Sciences, St. John's University, Jamaica, New York 11439.

Hirshfield I N

References (11)

11 references, click to expand

A model for three-point analysis of random general transduction.
Genetics. 1966 Aug;54(2):405-10 PMID: 5338813
High resolution two-dimensional electrophoresis of proteins.
J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
Metabolites influence control of lysine transfer ribonucleic acid synthetase formation in Escherichia coli K-12.
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1364-7 PMID: 805427
Two modes of metabolic regulation of lysyl-transfer ribonucleic acid synthetase in Escherichia coli K-12.
J Bacteriol. 1977 Aug;131(2):589-97 PMID: 328487
Multiple forms of lysyl-transfer ribonucleic acid synthetase in Escherichia coli.
J Bacteriol. 1981 Apr;146(1):345-51 PMID: 7012120
Structure and organization of the transfer ribonucleic acid genes of Escherichia coli K-12.
Microbiol Rev. 1985 Dec;49(4):379-97 PMID: 2419743
Gene for heat-inducible lysyl-tRNA synthetase (lysU) maps near cadA in Escherichia coli.
J Bacteriol. 1983 Feb;153(2):1066-8 PMID: 6337114
Convenient construction of recA deletion derivatives of Escherichia coli.
J Bacteriol. 1983 Oct;156(1):458-9 PMID: 6311804
Escherichia coli K-12 lysyl-tRNA synthetase mutant with a novel reversion pattern.
J Bacteriol. 1984 May;158(2):615-20 PMID: 6373723
Antisuppressor mutation in Escherichia coli defective in biosynthesis of 5-methylaminomethyl-2-thiouridine.
J Bacteriol. 1985 Jan;161(1):368-76 PMID: 3881393
Silver staining of proteins in polyacrylamide gels.
Anal Biochem. 1981 Nov 15;118(1):197-203 PMID: 6175245

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.169.11.5311-5313.1987

Published

1987-11-00

Pages

5311-3

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC213943

Subset

External Links

PubMed source DOI: 10.1128/jb.169.11.5311-5313.1987 PMC Full Text View journal details

Analysis Services

Mapping of the constitutive lysyl-tRNA synthetase gene of Escherichia coli K-12.

Contact

WeChat Official Account