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PMID: 1384058 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A fraction of the mRNA 5' cap-binding protein, eukaryotic initiation factor 4E, localizes to the nucleus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 20 ·1992-10-15 ·Pages 9612-6

Lejbkowicz F, Goyer C, Darveau A, Neron S, Lemieux R, Sonenberg N

Abstract

The 5' cap structure m7GpppN (where N is any nucleotide) is a ubiquitous feature of cellular eukaryotic mRNAs. The cap is multifunctional as it is involved in translation, nucleocytoplasmic transport, splicing, and stabilization of mRNA against 5' exonucleolytic degradation. The cap binding protein, eukaryotic initiation factor 4E (eIF-4E), is a translation initiation factor that binds to the cap structure and is part of a complex (eIF-4F) that promotes mRNA binding to ribosomes. Overexpression of eIF-4E in fibroblasts results in cell transformation. To test the hypothesis that some of the biological effects of eIF-4E might be effected by a nuclear function, we determined the cellular distribution of eIF-4E. By means of indirect immunofluorescence experiments using polyclonal and monoclonal antibodies against eIF-4E as well as transfected epitope-tagged eIF-4E, we demonstrate that a fraction of eIF-4E localizes to the nucleus. These results suggest that eIF-4E is also involved in a nuclear function.

MeSH Terms
Amino Acid Sequence Animals Blotting, Western Cell Compartmentation Cell Nucleus/metabolism Cells, Cultured Chlorocebus aethiops Epitopes Eukaryotic Initiation Factor-4E Fluorescent Antibody Technique In Vitro Techniques Molecular Sequence Data Nuclear Proteins/metabolism Peptide Initiation Factors/metabolism RNA Caps/metabolism
Chemicals
Epitopes Eukaryotic Initiation Factor-4E Nuclear Proteins Peptide Initiation Factors RNA Caps
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lejbkowicz F
Department of Biochemistry, McGill University, Montreal, PQ, Canada.
Goyer C
Darveau A
Neron S
Lemieux R
Sonenberg N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.20.9612
Published
1992-10-15
Pages
9612-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50182
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.20.9612 PMC Full Text View journal details
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