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PMID: 13866159 Published · ppublish English Journal Article

Glycine resistance in Agrobacterium tumefaciens.

Journal of bacteriology ·Vol. 83 ·1962-01-00 ·Pages 6-13

BEARDSLEY RE

Abstract

Beardsley, Robert E. (Manhattan College, New York, N. Y.). Glycine resistance in Agrobacterium tumefaciens. J. Bacteriol. 83:6-13. 1962.-The application of the fluctuation test of Luria and Delbrück to the distribution of glycine-resistant bacteria among cultures of Agrobacterium tumefaciens strain B6 indicates that resistance arises by mutation in the absence of glycine. On glycine-supplemented medium, additional resistant colonies arise during prolonged periods of incubation. Their appearance is proceded by L-form growth. In general, the number of generations over which glycine resistance is inherited in the absence of glycine is increased by serial transfers on the selection medium. In liquid medium containing glycine, sensitive bacteria form spheroplasts. Resistant bacteria continue to grow as rod forms. In the medium employed, spheroplasts are unstable.

Keywords
AGROBACTERIUM/metabolism GLYCINE/metabolism
MeSH Terms
Agrobacterium Agrobacterium tumefaciens Fabaceae Glycine/metabolism Mutation Rhizobium/metabolism
Chemicals
Glycine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
BEARDSLEY R E
References (16)
16 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1962-01-00
Pages
6-13
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC314780
Subset
OM
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