The mitotic apparatus. Fine structure of the isolated unit.

The Journal of cell biology ·Vol. 15 ·1962-11-00 ·Pages 279-87

KANE RE

Abstract

The fine structure of the mitotic apparatus isolated from the sea urchin egg has been investigated. The isolation was accomplished by lysis of metaphase eggs in a 1 M solution of hexanediol, buffered at pH 6. The fine structure of the isolated apparatus was studied after fixation with osmium tetroxide directly in the isolation medium. The spindle is composed of fine fibrils, approximately 20 mmicro in diameter, which appear tubular. Similar fibrils, radially oriented, are found in the aster. If the isolated mitotic apparatus is exposed to water at pH 6 before fixation, the structure is considerably modified. The most pronounced effects are an increase in the number of large membrane-bounded vesicles and in the amount of free granular material present. The conditions necessary for the fixation of the mitotic apparatus in dividing cells are discussed in the light of these observations on the isolated unit.

Keywords

CELL DIVISION

MeSH Terms

Animals Cell Division Cytoplasmic Granules Cytoskeleton Metaphase Sea Urchins Spindle Apparatus

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

KANE R E

References (14)

14 references, click to expand

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Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.15.2.279

Published

1962-11-00

Pages

279-87

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2106157

Subset

External Links

PubMed source DOI: 10.1083/jcb.15.2.279 PMC Full Text View journal details

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The mitotic apparatus. Fine structure of the isolated unit.

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