RHEUMATOID FACTOR PROPERTIES OF HYPERIMMUNE RABBIT SERA.

The Journal of experimental medicine ·Vol. 118 ·1963-11-01 ·Pages 827-44

CHRISTIAN CL

Abstract

The rheumatoid factor-like substance (RFLS) induced by hyperimmunization of rabbits with bacteria was consistently and exclusively associated with the macroglobulin fraction of the sera studied. The RFLS is separable from the bulk of serum proteins by zone centrifugation, DEAE cellulose chromatography, and gel filtration. Partially purified preparations of the RFLS were studied by immunoelectrophoretic analysis and analytical ultracentrifugation. Certain RFLS sera demonstrated selective reactivity with one or more rabbit isohemagglutinin antibodies. Pepsin treatment of rabbit gamma-globulin removed or greatly diminishes its capacity to react with the RFLS.

Keywords

AGGLUTINATION BACILLUS SUBTILIS ESCHERICHIA COLI EXPERIMENTAL LAB STUDY GAMMA GLOBULIN IMMUNE SERUMS IMMUNOELECTROPHORESIS PEPSIN RABBITS RHEUMATOID FACTOR SALMONELLA TYPHIMURIUM

MeSH Terms

Agglutination Animals Antibodies Bacillus subtilis Blood Proteins Escherichia coli Immune Sera Immunization Immunoelectrophoresis Pepsin A Rabbits Research Rheumatoid Factor Salmonella typhimurium gamma-Globulins

Chemicals

Antibodies Blood Proteins Immune Sera gamma-Globulins Rheumatoid Factor Pepsin A

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

CHRISTIAN C L

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24 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.118.5.827

Published

1963-11-01

Pages

827-44

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2137676

Subset

External Links

PubMed source DOI: 10.1084/jem.118.5.827 PMC Full Text View journal details

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