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PMID: 1409703 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Two members of a widely expressed subfamily of hormone-stimulated adenylyl cyclases.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 20 ·1992-10-15 ·Pages 9809-13

Premont RT, Chen J, Ma HW, Ponnapalli M, Iyengar R

Abstract

cDNA encoding a hormone- and guanine nucleotide-stimulated adenylyl cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] (type 6) from rat liver and kidney has been cloned and expressed. This enzyme is stimulated by forskolin, guanosine 5'-[gamma-thio]triphosphate, and isoproterenol plus GTP but is not stimulated by beta gamma subunits of guanine nucleotide-binding proteins. A second form (type 5), which is 75% similar to type 6, has also been cloned. Both types 5 and 6 cDNAs have multiple messages. PCR-based detection of the mRNA for the type 5 and 6 enzymes indicates that both are widely distributed. Homology analyses indicate at least four distinct subfamilies of guanine nucleotide stimulatory protein-regulated adenylyl cyclases. Types 5 and 6 enzymes define one distinct subfamily of mammalian adenylyl cyclases. Diversity of one guanine nucleotide-binding protein-regulated effector may allow different modes of regulation of cell-surface signal transmission.

MeSH Terms
Adenylyl Cyclases/classification,genetics Animals Base Sequence Cloning, Molecular Colforsin/pharmacology DNA/genetics Gene Expression/drug effects Isoproterenol/pharmacology Kidney/enzymology Liver/enzymology Membrane Proteins/classification,ultrastructure Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Polymerase Chain Reaction RNA, Messenger/genetics Rats Sequence Alignment Sequence Homology, Amino Acid Tissue Distribution
Chemicals
Membrane Proteins Oligodeoxyribonucleotides RNA, Messenger Colforsin DNA Adenylyl Cyclases Isoproterenol
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Premont R T
Department of Pharmacology, Mount Sinai School of Medicine, City University of New York, NY 10029.
Chen J
Ma H W
Ponnapalli M
Iyengar R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.20.9809
Published
1992-10-15
Pages
9809-13
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50222
Subset
IM
Grants
NCI NIH HHS · CA44998 · United States
NIDDK NIH HHS · DK38761 · United States
Databases
GENBANK
M96159, M96160
External Links
PubMed source DOI: 10.1073/pnas.89.20.9809 PMC Full Text View journal details
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