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PMID: 14273654 Published · ppublish English Journal Article

PREPARATION OF FLUORESCEIN ISOTHIOCYANATE-LABELED GAMMA-GLOBULIN BY DIALYSIS, GEL FILTRATION, AND IONEXCHANGE CHROMATOGRAPHY IN COMBINATION.

Journal of bacteriology ·Vol. 89 ·1965-03-00 ·Pages 734-9

DEDMON RE, HOLMES AW, DEINHARDT F

Abstract

Dedmon, Robert E. (Presbyterian-St. Luke's Hospital, Chicago, Ill.), Albert W. Holmes, and Friedrich Deinhardt. Preparation of fluorescein isothiocyanate-labeled gamma-globulin by dialysis, gel filtration, and ion-exchange chromatography in combination. J. Bacteriol. 89:734-739. 1965.-Antiviral immune gamma-globulins isolated from rabbit and guinea pig sera were labeled through dialysis membranes with fluorescein isothiocyanate and purified in several ways to eliminate nonspecific staining. Gel filtration of the conjugate with Sephadex G-25 coarse beads followed by column fractionation with diethylaminoethyl-Sephadex yielded consistently highly specific staining materials. Fluorescein-protein ratios varied between 1.0 and 4.0. This technique has proved to be simple and reliable, and is less time-consuming than previous techniques.

Keywords
CHROMATOGRAPHY DIALYSIS EXPERIMENTAL LAB STUDY FLUORESCEINS GAMMA GLOBULIN GEL FILTRATION THIOCYANATES
MeSH Terms
Animals Chromatography Chromatography, Gel Dextrans Dialysis Fluorescein Fluoresceins Guinea Pigs Isothiocyanates Rabbits Renal Dialysis Research Staining and Labeling Thiocyanates gamma-Globulins
Chemicals
Dextrans Fluoresceins Isothiocyanates Thiocyanates gamma-Globulins isothiocyanic acid sephadex Fluorescein
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
DEDMON R E
HOLMES A W
DEINHARDT F
References (19)
19 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.89.3.734-739.1965
Published
1965-03-00
Pages
734-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC277530
Subset
OM
External Links
PubMed source DOI: 10.1128/jb.89.3.734-739.1965 PMC Full Text View journal details
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