THE LYSIS OF CELL WALLS OF GROUP A STREPTOCOCCI BY STREPTOMYCES ALBUS ENZYME TREATED WITH DIISOPROPYL FLUOROPHOSPHATE. CHARACTERISTICS OF THE LYTIC REACTION AND THE SOLUBLE CELL WALL FRAGMENTS.

The Journal of experimental medicine ·Vol. 121 ·1965-05-01 ·Pages 771-92

SCHMIDT WC

Abstract

Diisopropyl fluorophosphate (DFP) effectively inhibited proteolytic activity in preparations of partially purified Streptomyces albus enzyme used to lyse cell walls of Group A streptococci. Lysis of non-trypsinized Group A cell walls with DFP-treated S. albus enzyme released a soluble protein fraction containing antigenic type-specific M protein, a carbohydrate fraction consisting of Group A and a small amount of A-variant polysaccharides, and a dialyzable fraction. The similarities of the products of DFP-treated S. albus enzyme lysis of streptococcal cell walls to those released by phage muralytic enzyme furnish additional evidence of the close relationship of these wall lysins. In view of small differences in electrophoretic mobility, immunodiffusion, and chemical composition, it is suggested that Group A streptococcal cell wall polysaccharide dissolved by DFP-S. albus enzyme consists of a spectrum of molecules having the same immunological determinants but differing in content of conjugated mucopeptide.

Keywords

BACTERIOLYSIS CHEMISTRY CHEMISTRY ANALYTICAL CHROMATOGRAPHY ELECTROPHORESIS ENZYMES EXPERIMENTAL LAB STUDY IMMUNOELECTROPHORESIS ISOFLUROPHATE PHARMACOLOGY POLYSACCHARIDES BACTERIAL STREPTOCOCCUS

MeSH Terms

Bacteriolysis Cell Wall Chemical Phenomena Chemistry Chemistry Techniques, Analytical Chromatography Electrophoresis Enzymes Fluorides Immunoelectrophoresis Isoflurophate Pharmacology Phosphates Polysaccharides, Bacterial Research Streptococcus Streptococcus pyogenes Streptomyces

Chemicals

Enzymes Phosphates Polysaccharides, Bacterial streptococcal polysaccharide group A Isoflurophate fluorophosphate Fluorides

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

SCHMIDT W C

References (22)

22 references, click to expand

The lysis of group A hemolytic streptococci by extracellular enzymes of Streptomyces albus. II. Nature of the cellular substrate attacked by the lytic enzymes.
J Exp Med. 1952 Dec;96(6):569-80 PMID: 13022851
STRUCTURE OF THE CELL WALL OF STAPHYLOCOCCUS AUREUS, STRAIN COPENHAGEN. I. PREPARATION OF FRAGMENTS BY ENZYMATIC HYDROLYSIS.
Biochemistry. 1963 Sep-Oct;2:1110-9 PMID: 14087369
Studies on the bacteriophages of hemolytic streptococci. II. Antigens released from the streptococcal cell wall by a phage-associated lysin.
J Exp Med. 1958 Dec 1;108(6):803-21 PMID: 13598814
Studies on L forms and protoplasts of group A streptococci. II. Chemical and immunological properties of the cell membrane.
J Exp Med. 1963 Mar 1;117:377-99 PMID: 13959531
Studies on the chemical structure of the streptococcal cell wall. I. The identification of a mucopeptide in the cell walls of groups A and A-variant streptococci.
J Exp Med. 1961 Jul 1;114:127-40 PMID: 13754097
Chemical structure and biosynthesis of bacterial cell walls.
Bacteriol Rev. 1963 Mar;27:18-55 PMID: 13942447
Identification of amino sugars.
Biochem J. 1959 Jul;72:479-86 PMID: 13813097
Studies of the bacterial cell wall. II. Methods of preparation and some properties of cell walls.
Biochim Biophys Acta. 1951 Jul;7(2):177-97 PMID: 14858403
THE DETERMINATION OF ANTIBODY TO GROUP A STREPTOCOCCAL POLYSACCHARIDE IN HUMAN SERA BY HEMAGGLUTINATION.
J Exp Med. 1965 May 1;121:793-806 PMID: 14280441
THE CELLULAR ANTIGENS OF GROUP A STREPTOCOCCI; IMMUNOELECTROPHORETIC STUDIES OF THE C, M, T, PGP, E4, F, AND E ANTIGENS OF SEROTYPE 17 STREPTOCOCCI.
J Exp Med. 1963 Oct 1;118:527-56 PMID: 14067904
The quantitative precipitin reaction of type 19 M protein antigen of group A streptococci and antistreptococcal rabbit sera.
J Immunol. 1957 Mar;78(3):178-84 PMID: 13429084
Variation in the group-specific carbohydrate of group A streptococci. I. Immunochemical studies on the carbohydrates of variant strains.
J Exp Med. 1955 Jul 1;102(1):11-28 PMID: 14392237
Variation in the group-specific carbohydrate of group A streptococci. II. Studies on the chemical basis for serological specificity of the carbohydrates.
J Exp Med. 1956 Nov 1;104(5):629-43 PMID: 13367334
SYMPOSIUM ON RELATIONSHIP OF STRUCTURE OF MICROORGANISMS TO THEIR IMMUNOLOGICAL PROPERTIES. IV. ANTIGENIC AND BIOCHEMICAL COMPOSITION OF HEMOLYTIC STREPTOCOCCAL CELL WALLS.
Bacteriol Rev. 1963 Dec;27:369-80 PMID: 14097346
The products of the partial acid hydrolysis of the mucopeptide from cell walls of Micrococcus lysodeikticus.
Biochem J. 1959 Aug;72:647-54 PMID: 14431858
The determination of glucosamine and galactosamine.
Biochem J. 1955 Dec;61(4):586-9 PMID: 13276341
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
The use of precipitin analysis in agar for the study of human streptococcal infections. III. The purification of some of the antigens detected by these methods.
J Exp Med. 1958 Sep 1;108(3):385-410 PMID: 13575674
Preparation and properties of type-specific M antigen isolated from a group A, type 1 hemolytic streptococcus.
J Exp Med. 1952 Jul;96(1):71-82 PMID: 14946330
Current knowledge of type-specific M antigens of group A streptococci.
J Immunol. 1962 Sep;89:307-13 PMID: 14461914
TYPING GROUP A HEMOLYTIC STREPTOCOCCI BY M PRECIPITIN REACTIONS IN CAPILLARY PIPETTES.
J Exp Med. 1943 Aug 1;78(2):127-33 PMID: 19871314
Inhibition of the proteinase and esterase activities of trypsin and chymotrypsin by diisopropyl fluorophosphate; crystallization of inhibited chymotrypsin.
J Biol Chem. 1949 May;179(1):189-99 PMID: 18119234

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.121.5.771

Published

1965-05-01

Pages

771-92

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2138002

Subset

External Links

PubMed source DOI: 10.1084/jem.121.5.771 PMC Full Text View journal details

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THE LYSIS OF CELL WALLS OF GROUP A STREPTOCOCCI BY STREPTOMYCES ALBUS ENZYME TREATED WITH DIISOPROPYL FLUOROPHOSPHATE. CHARACTERISTICS OF THE LYTIC REACTION AND THE SOLUBLE CELL WALL FRAGMENTS.

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