PREPARATION AND PROPERTIES OF THE PEPTIDE CHAINS OF NORMAL HUMAN 19 S GAMMA-GLOBULIN (IGM).

The Biochemical journal ·Vol. 95 ·1965-04-00 ·Pages 256-61

CHAPLIN H, COHEN S, PRESS EM

Abstract

1. A method is described for preparing pure samples of 19s gamma-globulin (IgM) from normal human serum by using successive steps of dialysis, density-gradient ultracentrifugation, chromatography on DEAE-cellulose, and gel filtration on Sephadex G-200. The yield of IgM (20-25mg./100ml. of serum) was equivalent to about one-quarter of that present in normal serum. 2. Analysis of the separated peptide chains of normal IgM and IgG (7s gamma-globulin) showed considerable differences in the amino acid composition of A chains from the two proteins; their respective B chains, on the other hand, were similar in composition. The carbohydrate of both proteins is confined almost entirely to the A chains; the IgM A chain contains about four times as much carbohydrate as the IgG A chain. 3. These findings support the view that the different classes of human immunoglobulin have B chains that are identical and A chains that are chemically distinct.

Keywords

BIOCHEMISTRY CHROMATOGRAPHY DIALYSIS GAMMA GLOBULIN 19S GEL FILTRATION IMMUNOCHEMISTRY IMMUNOELECTROPHORESIS PEPTIDES ULTRACENTRIFUGATION

MeSH Terms

Amino Acids Biochemical Phenomena Biochemistry Chromatography Chromatography, Gel Dialysis Humans Immunochemistry Immunoelectrophoresis Immunoglobulin G Immunoglobulin M Peptides Proteins Renal Dialysis Ultracentrifugation gamma-Globulins

Chemicals

Amino Acids Immunoglobulin G Immunoglobulin M Peptides Proteins gamma-Globulins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

CHAPLIN H

COHEN S

PRESS E M

References (28)

28 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj0950256

Published

1965-04-00

Pages

256-61

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1215202

Subset

External Links

PubMed source DOI: 10.1042/bj0950256 PMC Full Text View journal details

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PREPARATION AND PROPERTIES OF THE PEPTIDE CHAINS OF NORMAL HUMAN 19 S GAMMA-GLOBULIN (IGM).

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