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PMID: 1437564 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The basic RNA-binding domain of HIV-2 Tat contributes to preferential trans-activation of a TAR2-containing LTR.

Nucleic acids research ·Vol. 20 ·No. 20 ·1992-10-25 ·Pages 5465-72

Chang YN, Jeang KT

Abstract

Human immunodeficiency viruses HIV-1 and HIV-2 encode a Tat protein that trans-activates the respective viral genome through RNA targets (TAR1 and TAR2). Tat-1 and Tat-2 have considerable homology. However, an interesting biological observation has been that Tat-1 activates the HIV-1 and HIV-2 LTRs equally while Tat-2 activates the former, in comparison to the latter, poorly. Here, we present evidence that it is the TAR2 RNA target together with the basic RNA-binding protein domain of Tat-2 that dictate this non-reciprocity in trans-activation.

MeSH Terms
Amino Acid Sequence Arginine/metabolism Binding Sites/genetics Gene Products, tat/chemistry,genetics,metabolism HIV Long Terminal Repeat/genetics HIV-2/genetics HeLa Cells Humans Molecular Sequence Data Plasmids/genetics RNA, Viral/metabolism Radioligand Assay Recombinant Fusion Proteins/chemistry,metabolism Transcriptional Activation/genetics tat Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, tat RNA, Viral Recombinant Fusion Proteins tat Gene Products, Human Immunodeficiency Virus Arginine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chang Y N
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892.
Jeang K T
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33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.20.5465
Published
1992-10-25
Pages
5465-72
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334357
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/20.20.5465 PMC Full Text View journal details
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