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PMID: 14517321 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pex11-related proteins in peroxisome dynamics: a role for the novel peroxin Pex27p in controlling peroxisome size and number in Saccharomyces cerevisiae.

Molecular biology of the cell ·Vol. 14 ·No. 10 ·2003-10-00 ·Pages 4089-102

Tam YY, Torres-Guzman JC, Vizeacoumar FJ, Smith JJ, Marelli M, Aitchison JD, Rachubinski RA

Abstract

Transcriptome profiling identified the gene PEX25 encoding Pex25p, a peroxisomal membrane peroxin required for the regulation of peroxisome size and maintenance in Saccharomyces cerevisiae. Pex25p is related to a protein of unknown function encoded by the open reading frame, YOR193w, of the S. cerevisiae genome. Yor193p is a peripheral peroxisomal membrane protein that exhibits high sequence similarity not only to Pex25p but also to the peroxisomal membrane peroxin Pex11p. Unlike Pex25p and Pex11p, Yor193p is constitutively expressed in wild-type cells grown in oleic acid-containing medium, the metabolism of which requires intact peroxisomes. Cells deleted for the YOR193w gene show a few enlarged peroxisomes. Peroxisomes are greatly enlarged in cells harboring double deletions of the YOR193w and PEX25 genes, the YOR193w and PEX11 genes, and the PEX25 and PEX11 genes. Yeast two-hybrid analyses showed that Yor193p interacts with Pex25p and itself, Pex25p interacts with Yor193p and itself, and Pex11p interacts only with itself. Overexpression of YOR193w, PEX25, or PEX11 led to peroxisome proliferation and the formation of small peroxisomes. Our data suggest a role for Yor193p, renamed Pex27p, in controlling peroxisome size and number in S. cerevisiae.

MeSH Terms
Amino Acid Sequence Gene Expression Regulation, Fungal Intracellular Membranes/metabolism Membrane Proteins/genetics,metabolism Microscopy, Electron Molecular Sequence Data Oleic Acid/pharmacology Peroxins Peroxisomes/genetics,metabolism Protein Binding Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Sequence Homology, Amino Acid Two-Hybrid System Techniques
Chemicals
Membrane Proteins PEX11 protein, S cerevisiae PEX25 protein, S cerevisiae Peroxins Pex27 protein, S cerevisiae Saccharomyces cerevisiae Proteins Oleic Acid
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Tam Yuen Yi C
Department of Cell Biology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.
Torres-Guzman Juan C
Vizeacoumar Franco J
Smith Jennifer J
Marelli Marcello
Aitchison John D
Rachubinski Richard A
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e03-03-0150
Published
2003-10-00
Epub
2003-00-18
Pages
4089-102
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC207002
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e03-03-0150 PMC Full Text View journal details
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