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PMID: 12135984 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcriptome profiling to identify genes involved in peroxisome assembly and function.

The Journal of cell biology ·Vol. 158 ·No. 2 ·2002-07-22 ·Pages 259-71

Smith JJ, Marelli M, Christmas RH, Vizeacoumar FJ, Dilworth DJ, Ideker T, Galitski T, Dimitrov K, Rachubinski RA, Aitchison JD

Abstract

Yeast cells were induced to proliferate peroxisomes, and microarray transcriptional profiling was used to identify PEX genes encoding peroxins involved in peroxisome assembly and genes involved in peroxisome function. Clustering algorithms identified 224 genes with expression profiles similar to those of genes encoding peroxisomal proteins and genes involved in peroxisome biogenesis. Several previously uncharacterized genes were identified, two of which, YPL112c and YOR084w, encode proteins of the peroxisomal membrane and matrix, respectively. Ypl112p, renamed Pex25p, is a novel peroxin required for the regulation of peroxisome size and maintenance. These studies demonstrate the utility of comparative gene profiling as an alternative to functional assays to identify genes with roles in peroxisome biogenesis.

MeSH Terms
Gene Expression Profiling Gene Expression Regulation, Fungal Genes, Fungal Peroxisomes/genetics,metabolism Saccharomyces cerevisiae/genetics Transcriptional Activation
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Smith Jennifer J
The Institute for Systems Biology, 1441 N. 34th Street, Seattle, WA 98103-8904, USA.
Marelli Marcello
Christmas Rowan H
Vizeacoumar Franco J
Dilworth David J
Ideker Trey
Galitski Timothy
Dimitrov Krassen
Rachubinski Richard A
Aitchison John D
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200204059
Published
2002-07-22
Epub
2002-00-22
Pages
259-71
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2173120
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200204059 PMC Full Text View journal details
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