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PMID: 8858165 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Pex13p is an SH3 protein of the peroxisome membrane and a docking factor for the predominantly cytoplasmic PTs1 receptor.

The Journal of cell biology ·Vol. 135 ·No. 1 ·1996-10-00 ·Pages 85-95

Gould SJ, Kalish JE, Morrell JC, Bjorkman J, Urquhart AJ, Crane DI

Abstract

Import of newly synthesized PTS1 proteins into the peroxisome requires the PTS1 receptor (Pex5p), a predominantly cytoplasmic protein that cycles between the cytoplasm and peroxisome. We have identified Pex13p, a novel integral peroxisomal membrane from both yeast and humans that binds the PTS1 receptor via a cytoplasmically oriented SH3 domain. Although only a small amount of Pex5p is bound to peroxisomes at steady state (< 5%), loss of Pex13p further reduces the amount of peroxisome-associated Pex5p by approximately 40-fold. Furthermore, loss of Pex13p eliminates import of peroxisomal matrix proteins that contain either the type-1 or type-2 peroxisomal targeting signal but does not affect targeting and insertion of integral peroxisomal membrane proteins. We conclude that Pex13p functions as a docking factor for the predominantly cytoplasmic PTS1 receptor.

MeSH Terms
Acetyl-CoA C-Acetyltransferase/metabolism Amino Acid Sequence Biological Transport Catalase/metabolism Cell Adhesion Molecules/metabolism Cell Line Cell Membrane/chemistry Cloning, Molecular Cytoplasm/metabolism Fibroblasts Genes/genetics Genes, Fungal/genetics Humans Intracellular Membranes/chemistry Membrane Proteins/analysis,genetics,metabolism,physiology Microbodies/chemistry,metabolism Molecular Sequence Data Peroxisomal Targeting Signal 2 Receptor Peroxisome-Targeting Signal 1 Receptor Pichia/genetics Receptors, Cytoplasmic and Nuclear/metabolism Sequence Analysis, DNA src Homology Domains
Chemicals
Cell Adhesion Molecules Membrane Proteins PEX13 protein, human Peroxisomal Targeting Signal 2 Receptor Peroxisome-Targeting Signal 1 Receptor Receptors, Cytoplasmic and Nuclear Catalase Acetyl-CoA C-Acetyltransferase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Gould S J
Department of Biological Chemistry, Johns Hopkins University School of Medicine, Kennedy Krieger Institute, Baltimore, Maryland 21205, USA. [email protected]
Kalish J E
Morrell J C
Bjorkman J
Urquhart A J
Crane D I
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.135.1.85
Email
[email protected]
Published
1996-10-00
Pages
85-95
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2121023
Subset
IM
Grants
NIDDK NIH HHS · DK45787 · United States
Databases
GENBANK
U59222, U71374
External Links
PubMed source DOI: 10.1083/jcb.135.1.85 PMC Full Text View journal details
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