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PMID: 8265627 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

PAS10 is a tetratricopeptide-repeat protein that is essential for the import of most matrix proteins into peroxisomes of Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 24 ·1993-12-15 ·Pages 11782-6

Van der Leij I, Franse MM, Elgersma Y, Distel B, Tabak HF

Abstract

pas mutants of Saccharomyces cerevisiae are disturbed in peroxisome assembly (pas) and proliferation. Here we report the characterization of the PAS10 gene and its product (PAS10) that is essential for the import of a large subset of proteins into the peroxisomal matrix. PAS10, a protein of 69 kDa, is a member of the tetratricopeptide repeat, or snap helix, protein family, characterized by several direct repeats of a degenerate 34-amino acid motif (Sikorski, R. S., Boguski, M. S., Goebl, M. & Hieter, P. (1990) Cell 60, 307-317). Other members of this family are MAS70 (S. cerevisiae) and MOM72 (Neurospora crassa), which are mitochondrial receptors for protein import. A pas10 null mutant accumulates peroxisomal, leaflet-like membrane structures and exhibits deficient import of a number of peroxisomal matrix enzymes, particularly of proteins with an SKL-like import signal. In contrast, 3-ketoacyl-CoA thiolase associated with these membranes is resistant in vitro to degradation by proteinase K, indicating true protein import. These results suggest that PAS10 is an essential component of a peroxisomal import machinery which mediates the translocation of a specific subset of proteins to the peroxisomal matrix with an SKL-like import signal.

Related Genes
PAS10
MeSH Terms
Amino Acid Sequence Base Sequence Carrier Proteins/biosynthesis,genetics,metabolism Cloning, Molecular Conserved Sequence DNA, Fungal/isolation & purification Electrophoresis, Polyacrylamide Gel Fungal Proteins/biosynthesis,genetics,metabolism Genes, Fungal Membrane Transport Proteins Microbodies/metabolism Molecular Sequence Data Open Reading Frames Peroxisome-Targeting Signal 1 Receptor Plasmids RNA, Messenger/biosynthesis,isolation & purification Repetitive Sequences, Nucleic Acid Restriction Mapping Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins
Chemicals
Carrier Proteins DNA, Fungal Fungal Proteins Membrane Transport Proteins PEX5 protein, S cerevisiae Peroxisome-Targeting Signal 1 Receptor RNA, Messenger Saccharomyces cerevisiae Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Van der Leij I
E. C. Slater Institute, University of Amsterdam, The Netherlands.
Franse M M
Elgersma Y
Distel B
Tabak H F
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.24.11782
Published
1993-12-15
Pages
11782-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48068
Subset
IM
Databases
GENBANK
L23076
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1073/pnas.90.24.11782 PMC Full Text View journal details
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