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PMID: 3554235 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Firefly luciferase is targeted to peroxisomes in mammalian cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 10 ·1987-05-00 ·Pages 3264-8

Keller GA, Gould S, Deluca M, Subramani S

Abstract

Although several enzymes known to reside in peroxisomes have been studied extensively, no cis-acting amino acid sequences involved in the transport of these proteins to peroxisomes have been described. As a first step towards the determination of a putative peroxisomal targeting sequence, we have expressed the cDNA encoding the firefly luciferase [Photinus-luciferin:oxygen 4-oxidoreductase (decarboxylating, ATP-hydrolyzing), EC 1.13.12.7] in monkey kidney cells and found that the product of the gene is transported to peroxisomes. Luciferase is derived from the firefly (Photinus pyralis) and is synthesized and stored in the cells of the firefly's lantern organ, where it is also found in peroxisomes. The fact that this protein is similarly targeted in cells from such different organisms suggests that the process of protein transport to peroxisomes has been highly conserved through evolution.

MeSH Terms
Animals Biological Transport Cell Line Coleoptera/enzymology,genetics DNA/metabolism Fluorescent Antibody Technique Genes Luciferases/genetics,metabolism Microbodies/metabolism,ultrastructure Microscopy, Electron Transcription, Genetic
Chemicals
DNA Luciferases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Keller G A
Gould S
Deluca M
Subramani S
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.10.3264
Published
1987-05-00
Pages
3264-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304849
Subset
IM
Grants
NCI NIH HHS · CA 01062 · United States
NIGMS NIH HHS · GM 07313 · United States
NHLBI NIH HHS · HL 14197 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.10.3264 PMC Full Text View journal details
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