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PMID: 6594687 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Synthesis of a major integral membrane polypeptide of rat liver peroxisomes on free polysomes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 22 ·1984-11-00 ·Pages 7127-31

Fujiki Y, Rachubinski RA, Lazarow PB

Abstract

The manner of synthesis and assembly of the peroxisomal membrane proteins is unknown. Understanding these processes is essential to an understanding of the formation of the organelle. We have investigated the biogenesis of the previously identified major 21.7-kDa integral peroxisomal membrane polypeptide [Fujiki, Y., Fowler, S., Shio, H., Hubbard, A. L. & Lazarow, P. B. (1982) J. Cell Biol. 93, 103-110]. This protein was purified to apparent homogeneity and used to elicit a rabbit antiserum. In immunoblotting analysis, antibody bound only to the 22-kDa membrane polypeptide present exclusively in peroxisomal membranes. Total rat liver RNA was translated in a nuclease-treated rabbit reticulocyte cell-free protein-synthesizing system. The in vitro translation product, isolated by means of the antibody and Staphylococcus aureus cells, comigrated with the mature 22-kDa polypeptide in NaDodSO4/PAGE. Analysis of the translation products of RNAs from free and membrane-bound polysomes indicated that the mRNA for the 22-kDa membrane polypeptide is found predominantly in free polysomes. The results imply post-translational insertion of the membrane polypeptide into the peroxisomal membrane without proteolytic processing and suggest that peroxisomes, like mitochondria and chloroplasts, form by fission from preexisting organelles.

MeSH Terms
Animals Cytoplasm/metabolism Intracellular Membranes/metabolism Liver/metabolism Membrane Proteins/biosynthesis,immunology,isolation & purification Microbodies/analysis,metabolism Molecular Weight Polyribosomes/metabolism Rats
Chemicals
Membrane Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fujiki Y
Rachubinski R A
Lazarow P B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.22.7127
Published
1984-11-00
Pages
7127-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392090
Subset
IM
Grants
NIADDK NIH HHS · AM 19394 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.22.7127 PMC Full Text View journal details
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