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PMID: 7358795 Published · ppublish English Journal Article

Synthesis of rat liver microsomal cytochrome b5 by free ribosomes.

The Journal of cell biology ·Vol. 84 ·No. 3 ·1980-03-00 ·Pages 705-16

Rachubinski RA, Verma DP, Bergeron JJ

Abstract

Free and membrane-bound polyribosomes were separated from liver homogenates and characterized by electron microscopy. Using the wheat germ cell-free translation system, total translation products of poly A+RNA extracted from free polyribosomes (poly A+RNAf) showed some correlation to total liver cytosol proteins. In contrast, translation products of poly A+RNA from membrane-bound polyribosomes (poly A+RNAmb) showed some similarity to rat serum. Antibody to purified rat serum albumin immunoprecipitated from only the translation products of poly A+RNAmb a single polypeptide of mol wt 68,000. i.e., 3,000 greater than secreted serum albumin. In contrast, antibody to detergent-extracted cytochrome b5 immunoprecipitated from only the translation products of poly A+RNAf a single polypeptide of mol wt 17,500, identical to that of microsomal cytochrome b5. A consideration of the known properties of cytochrome b5 is consistent with an exclusive site of synthesis on free ribosomes.

MeSH Terms
Albumins/biosynthesis Animals Cell Fractionation Cytochromes Cytochromes b5 Microsomes, Liver Molecular Weight Polyribosomes/metabolism Protein Biosynthesis RNA/metabolism Rats
Chemicals
Albumins Cytochromes RNA Cytochromes b5
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rachubinski R A
Verma D P
Bergeron J J
References (66)
66 references, click to expand
  1. Coupled cell-free synthesis, segregation, and core glycosylation of a secretory protein.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2338-42 PMID: 276877
  2. Regulation of synthesis of hepatic fatty acid synthetase: polysomal translation in a cell-free system.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4366-70 PMID: 1060113
  3. Proteolytic cleavage of horse liver cytochrome b5. Primary structure of the heme-containing moiety.
    J Biol Chem. 1976 Nov 10;251(21):6767-74 PMID: 977596
  4. Electron microscopic examination of subcellular fractions. I. The preparation of representative samples from suspensions of particles.
    J Cell Biol. 1967 Jan;32(1):181-91 PMID: 10976209
  5. B-type cytochromes in plasma membranes isolated from rat liver, in comparison with those of endomembranes.
    J Cell Biol. 1979 Jan;80(1):37-52 PMID: 106058
  6. Rat liver preproalbumin: in vitro synthesis and partial amino acid sequence.
    Proc Natl Acad Sci U S A. 1977 Apr;74(4):1358-62 PMID: 266178
  7. Golgi fractions prepared from rat liver homogenates. II. Biochemical characterization.
    J Cell Biol. 1973 Oct;59(1):73-88 PMID: 4356572
  8. The interaction of NADH-cytochrome b5 reductase and cytochrome b5 bound to egg lecithin liposomes.
    J Biol Chem. 1975 Jul 25;250(14):5713-8 PMID: 167022
  9. Biosynthesis of serum proteins and ferritin by free and attached ribosomes of rat liver.
    J Biol Chem. 1969 Aug 25;244(16):4308-15 PMID: 5806579
  10. Purification of cytochrome b5.
    Methods Enzymol. 1978;52:97-101 PMID: 672659
  11. Covalent structure of the membranous segment of horse cytochrome b5. Chemical cleavage of the native hemoprotein.
    J Biol Chem. 1977 Dec 10;252(23):8549-53 PMID: 562879
  12. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  13. Rat liver pre-proalbumin: complete amino acid sequence of the pre-piece. Analysis of the direct translation product of albumin messenger RNA.
    J Biol Chem. 1977 Oct 10;252(19):6846-55 PMID: 893447
  14. Preferential synthesis of ferritin and albumin by different populations of liver polysomes.
    Science. 1969 May 2;164(3879):584-5 PMID: 5781696
  15. Evidence for the participation of the Golgi apparatus in the intracellular transport of nascent albumin in the liver cell.
    J Cell Biol. 1970 Dec;47(3):555-67 PMID: 5497537
  16. Rat hepatoma 5123TC albumin mRNA directs the synthesis of pre-proalbumin identical to rat liver pre-proalbumin.
    Biochem Biophys Res Commun. 1977 Aug 22;77(4):1224-30 PMID: 197957
  17. The nonpolar peptide segment of cytochrome b5. Binding to phospholipid vesicles and identification of the fluorescent tryptophanyl residue.
    J Biol Chem. 1978 Nov 25;253(22):8198-202 PMID: 711745
  18. Studies on the synthesis and intracellular transport of lipoprotein particles in rat liver.
    J Cell Biol. 1975 Feb;64(2):356-77 PMID: 1117030
  19. Quantification of Coomassie Blue stained proteins in polyacrylamide gels based on analyses of eluted dye.
    Anal Biochem. 1975 Feb;63(2):595-602 PMID: 47719
  20. Golgi fractions prepared from rat liver homogenates. I. Isolation procedure and morphological characterization.
    J Cell Biol. 1973 Oct;59(1):45-72 PMID: 4356571
  21. Biogenesis of plasmalemmal glycoproteins. Intracellular site of synthesis of mouse liver plasmalemmal 5'-nucleotidase as determined by the sub-cellular location of messenger RNA coding for 5'-nucleotidase.
    Biochim Biophys Acta. 1975 Oct 15;407(3):325-37 PMID: 810623
  22. Recent developments in the measurement of nucleic acids in biological materials. A supplementary review.
    Analyst. 1966 Feb;91(79):78-88 PMID: 5323520
  23. Conversion of proalbumin into serum albumin in the secretory vesicles of rat liver.
    Biochem Biophys Res Commun. 1976 Sep 7;72(1):319-26 PMID: 985475
  24. Amino acid sequence of rabbit liver microsomal cytochrome b5.
    J Biol Chem. 1970 Oct 10;245(19):4863-74 PMID: 5506260
  25. Leghemoglobin biosynthesis in soybean root nodules. Characterization of the nascent and released peptides and the relative rate of synthesis of the major leghemoglobins.
    Biochemistry. 1979 Feb 6;18(3):476-83 PMID: 570410
  26. Biogenesis of endoplasmic reticulum membrane in rat liver cells. I. Intracellular sites of synthesis of cytochrome b5 and NADPH-cytochrome c reductase.
    J Biochem. 1977 Dec;82(6):1541-9 PMID: 413826
  27. Controlled proteolysis of nascent polypeptides in rat liver cell fractions. I. Location of the polypeptides within ribosomes.
    J Cell Biol. 1970 Apr;45(1):130-45 PMID: 5458992
  28. The binding of cytochrome b 5 to liver microsomes.
    J Biol Chem. 1972 Nov 25;247(22):7188-94 PMID: 4404746
  29. Glycosylation of rat liver cytochrome b5 on the ribosomal level.
    Biochem Biophys Res Commun. 1978 Oct 16;84(3):572-80 PMID: 718700
  30. Correction of the amino acid sequence of calf liver microsomal cytochrome b5.
    J Biol Chem. 1969 Dec 25;244(24):6617-8 PMID: 5391285
  31. Immunological similarity of the NADH-cytochrome c electron transport system in microsomes, Golgi complex and mitochondrial outer membrane of rat liver cells.
    FEBS Lett. 1976 Apr 1;63(2):231-4 PMID: 177314
  32. Cytochrome b5 and P-450 in liver cell fractions.
    Biochim Biophys Acta. 1971 Feb 2;225(2):194-200 PMID: 4324133
  33. Methylation of TMV RNA.
    Biochem Biophys Res Commun. 1975 Feb 17;62(4):798-807 PMID: 1091268
  34. Nuclear membranes from mammalian liver. I. Isolation procedure and general characterization.
    J Cell Biol. 1970 Aug;46(2):379-95 PMID: 4317731
  35. The biosynthesis of rat serum albumin. IV. Apparent passage of albumin through the Golgi apparatus during secretion.
    J Biol Chem. 1971 Jan 10;246(1):240-4 PMID: 5541766
  36. Regulation of RNA synthesis in plant cell culture: delayed synthesis of ribosomal RNA during transition from the stationary phase to active growth.
    Dev Biol. 1973 Jan;30(1):104-14 PMID: 4697743
  37. An efficient mRNA-dependent translation system from reticulocyte lysates.
    Eur J Biochem. 1976 AUG 1;67(1):247-56 PMID: 823012
  38. Studies on free and membrane-bound ribosomes in rat liver. I. Distribution as related to total cellular RNA.
    J Mol Biol. 1967 Jun 14;26(2):279-92 PMID: 6034775
  39. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.
    J Cell Biol. 1975 Dec;67(3):835-51 PMID: 811671
  40. Actin antibody: the specific visualization of actin filaments in non-muscle cells.
    Proc Natl Acad Sci U S A. 1974 Jun;71(6):2268-72 PMID: 4210210
  41. A new method using 'proteinase K' to prevent mRNA degradation during isolation from HeLa cells.
    Biochem Biophys Res Commun. 1971 Jul 16;44(2):513-9 PMID: 5159785
  42. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.
    Eur J Biochem. 1975 Aug 15;56(2):335-41 PMID: 1175627
  43. Bacteriophage T7.
    Science. 1972 Apr 28;176(4033):367-76 PMID: 4554613
  44. Primary structure of the membranous segment of cytochrome b5.
    Proc Natl Acad Sci U S A. 1977 Sep;74(9):3725-9 PMID: 269425
  45. Cytochrome b5 and CO-binding cytochromes in the Golgi membranes of mammalian livers.
    Biochem Biophys Res Commun. 1970 Jul 27;40(2):297-305 PMID: 5474785
  46. Cytochromes in bovine liver nuclear membranes.
    Biochem Biophys Res Commun. 1971 Jun 4;43(5):1017-23 PMID: 4398131
  47. Binding and uptake of 125I-insulin into rat liver hepatocytes and endothelium. An in vivo radioautographic study.
    J Cell Biol. 1979 Feb;80(2):427-43 PMID: 457750
  48. Colchicine inhibition of plasma protein release from rat hepatocytes.
    J Cell Biol. 1975 Jul;66(1):42-59 PMID: 1141379
  49. Two fractions of rough endoplasmic reticulum from rat liver. II. Cytoplasmic messenger RNA's which code for albumin and mitochondrial proteins are distributed differently between the two fractions.
    J Cell Biol. 1977 Mar;72(3):726-43 PMID: 838773
  50. Binding of cytochrome b5 to membranes of isolated subcellular organelles from rat liver.
    J Cell Biol. 1978 Nov;79(2 Pt 1):291-313 PMID: 721892
  51. Passage of serum-destined proteins through the Golgi apparatus of rat liver. An examination of heavy and light Golgi fractions.
    J Cell Biol. 1978 Jan;76(1):87-97 PMID: 618900
  52. A Hemochromogen Component of Liver Microsomes.
    Proc Natl Acad Sci U S A. 1952 Jan;38(1):19-25 PMID: 16589046
  53. Turnover of constituents of the endoplasmic reticulum membranes of rat hepatocytes.
    J Biol Chem. 1967 May 25;242(10):2389-96 PMID: 4381697
  54. Purification and properties of rat liver microsomal stearyl coenzyme A desaturase.
    Proc Natl Acad Sci U S A. 1974 Nov;71(11):4565-9 PMID: 4373719
  55. A comparison of microbody membranes with microsomes and mitochondria from plant and animal tissue.
    Arch Biochem Biophys. 1972 Sep;152(1):199-215 PMID: 4342107
  56. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  57. Tropomyosin antibody: the specific localization of tropomyosin in nonmuscle cells.
    J Cell Biol. 1975 Jun;65(3):549-61 PMID: 1094020
  58. Cell-free translation of the messenger RNA coding for the alpha subunit of thyroid-stimulating hormone.
    J Biol Chem. 1978 Nov 25;253(22):7985-8 PMID: 711729
  59. Cell-free synthesis of fish preproinsulin, and processing by heterologous mammalian microsomal membranes.
    Proc Natl Acad Sci U S A. 1977 May;74(5):2059-63 PMID: 325565
  60. Electron microscope examination of subcellular fractions. 3. Quantitative analysis of the microsomal fraction isolated from rat liver.
    J Cell Biol. 1971 Oct;51(1):52-71 PMID: 4329524
  61. Mechanism of cytochrome b5 binding to phosphatidylcholine vesicles.
    J Biol Chem. 1979 Jun 25;254(12):5015-9 PMID: 447630
  62. A method for visualizing and quantifying the total complement of free and membrane-bound ribosomes in rat liver.
    Anal Biochem. 1979 Jan 15;92(2):305-13 PMID: 443531
  63. A procedure for the quantitative recovery of homogeneous populations of undegraded free and bound polysomes from rat liver.
    Biochemistry. 1976 Apr 20;15(8):1704-12 PMID: 1268192
  64. An electron-transport system associated with the outer membrane of liver mitochondria. A biochemical and morphological study.
    J Cell Biol. 1967 Feb;32(2):415-38 PMID: 10976232
  65. Effect of hypophysectomy on the synthesis of rat liver albumin.
    J Biol Chem. 1976 Jun 25;251(12):3768-73 PMID: 932005
  66. A form of cytochrome b5 that contains an additional hydrophobic sequence of 40 amino acid residues.
    Proc Natl Acad Sci U S A. 1971 May;68(5):1042-6 PMID: 4995819
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.84.3.705
Published
1980-03-00
Pages
705-16
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2110564
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.84.3.705 PMC Full Text View journal details
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