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PMID: 2568633 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of peroxisome-deficient mutants of Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 14 ·1989-07-00 ·Pages 5419-23

Erdmann R, Veenhuis M, Mertens D, Kunau WH

Abstract

Two mutants of Saccharomyces cerevisiae affected in peroxisomal assembly (pas mutants) have been isolated and characterized. Each strain contains a single mutation that results in (i) the inability to grow on oleic acid, (ii) accumulation of peroxisomal matrix enzymes in the cytosol, and (iii) absence of detectable peroxisomes at the ultrastructural level. These lesions (pas1-1 and pas2) are shown to be nonallelic and recessive. Crossing of pas1-1 and pas2 strains resulted in diploid cells that had regained the ability to grow on oleic acid as sole carbon source and to form peroxisomes. These pas mutants may provide useful tools for future studies on the molecular mechanisms involved in peroxisomal assembly.

MeSH Terms
Acetyl-CoA C-Acetyltransferase/analysis Catalase/analysis Fumarate Hydratase/analysis Genetic Complementation Test Microbodies/ultrastructure Microscopy, Electron Mutation Oleic Acid Oleic Acids/metabolism Saccharomyces cerevisiae/enzymology,genetics,ultrastructure
Chemicals
Oleic Acids Oleic Acid Catalase Acetyl-CoA C-Acetyltransferase Fumarate Hydratase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Erdmann R
Institute of Physiological Chemistry, Ruhr-University, Bochum, Federal Republic of Germany.
Veenhuis M
Mertens D
Kunau W H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.14.5419
Published
1989-07-00
Pages
5419-23
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297634
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.86.14.5419 PMC Full Text View journal details
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