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PMID: 8858166 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The SH3 domain of the Saccharomyces cerevisiae peroxisomal membrane protein Pex13p functions as a docking site for Pex5p, a mobile receptor for the import PTS1-containing proteins.

The Journal of cell biology ·Vol. 135 ·No. 1 ·1996-10-00 ·Pages 97-109

Elgersma Y, Kwast L, Klein A, Voorn-Brouwer T, van den Berg M, Metzig B, America T, Tabak HF, Distel B

Abstract

We identified a Saccharomyces cerevisiae peroxisomal membrane protein, Pex13p, that is essential for protein import. A point mutation in the COOH-terminal Src homology 3 (SH3) domain of Pex13p inactivated the protein but did not affect its membrane targeting. A two-hybrid screen with the SH3 domain of Pex13p identified Pex5p, a receptor for proteins with a type I peroxisomal targeting signal (PTS1), as its ligand. Pex13p SH3 interacted specifically with Pex5p in vitro. We determined, furthermore, that Pex5p was mainly present in the cytosol and only a small fraction was associated with peroxisomes. We therefore propose that Pex13p is a component of the peroxisomal protein import machinery onto which the mobile Pex5p receptor docks for the delivery of the selected PTS1 protein.

MeSH Terms
3-Hydroxyacyl CoA Dehydrogenases/metabolism Acetyl-CoA C-Acetyltransferase/analysis Amino Acid Sequence Base Sequence Biological Transport Catalase/metabolism Cloning, Molecular Cytosol/chemistry Fibroblasts Genes, Fungal/genetics Humans Intracellular Membranes/chemistry Ligands Membrane Proteins/analysis,genetics,metabolism Microbodies/chemistry,metabolism Molecular Sequence Data Peroxisome-Targeting Signal 1 Receptor Point Mutation Receptors, Cytoplasmic and Nuclear/analysis,genetics,metabolism Recombinant Fusion Proteins/metabolism Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Deletion src Homology Domains/genetics
Chemicals
Ligands Membrane Proteins PEX13 protein, S cerevisiae PEX13 protein, human Peroxisome-Targeting Signal 1 Receptor Receptors, Cytoplasmic and Nuclear Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins 3-Hydroxyacyl CoA Dehydrogenases Catalase Acetyl-CoA C-Acetyltransferase
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Elgersma Y
Department of Biochemistry, Academic Medical Centre, Amsterdam, The Netherlands.
Kwast L
Klein A
Voorn-Brouwer T
van den Berg M
Metzig B
America T
Tabak H F
Distel B
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.135.1.97
Published
1996-10-00
Pages
97-109
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2121018
Subset
IM
Databases
GENBANK
S82971
External Links
PubMed source DOI: 10.1083/jcb.135.1.97 PMC Full Text View journal details
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