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PMID: 1651504 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cu,Zn superoxide dismutase is a peroxisomal enzyme in human fibroblasts and hepatoma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 16 ·1991-08-15 ·Pages 7381-5

Keller GA, Warner TG, Steimer KS, Hallewell RA

Abstract

The intracellular localization of Cu,Zn superoxide dismutase (superoxide:superoxide oxidoreductase, EC 1.15.1.1) has been examined by immunofluorescence using four monoclonal anti-Cu,Zn superoxide dismutase antibodies raised against a recombinant human Cu,Zn superoxide dismutase derivative produced and purified from Escherichia coli. Colocalization with catalase, a peroxisomal matrix enzyme, was used to demonstrate the peroxisomal localization of Cu,Zn superoxide dismutase in human fibroblasts and hepatoma cells. In the fibroblasts of Zellweger syndrome patients, the enzyme is not transported to the peroxisomal ghosts but, like catalase, remains in the cytoplasm. In addition, immunocryoelectron microscopy of yeast cells expressing human Cu,Zn superoxide dismutase showed that the enzyme is translocated to the peroxisomes.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal Carcinoma, Hepatocellular/enzymology Fibroblasts/enzymology,ultrastructure Fluorescent Antibody Technique Humans Liver Neoplasms/enzymology Mice Mice, Inbred BALB C/immunology Microbodies/enzymology,ultrastructure Microscopy, Immunoelectron Molecular Sequence Data Recombinant Proteins/analysis,immunology Saccharomyces cerevisiae/genetics Superoxide Dismutase/analysis,immunology Zellweger Syndrome/enzymology,pathology
Chemicals
Antibodies, Monoclonal Recombinant Proteins Superoxide Dismutase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Keller G A
Genentech Inc., South San Francisco, CA 94080.
Warner T G
Steimer K S
Hallewell R A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.16.7381
Published
1991-08-15
Pages
7381-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52299
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.88.16.7381 PMC Full Text View journal details
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