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PMID: 1454813 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Structural analysis of the human interferon gamma receptor: a small segment of the intracellular domain is specifically required for class I major histocompatibility complex antigen induction and antiviral activity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 23 ·1992-12-01 ·Pages 11317-21

Cook JR, Jung V, Schwartz B, Wang P, Pestka S

Abstract

Mutations of the human interferon gamma (IFN-gamma) receptor intracellular domain have permitted us to define a restricted region of that domain as necessary for both induction of class I major histocompatibility complex antigen by IFN-gamma and protection against encephalomyocarditis virus. This region consists of five amino acids (YDKPH), all of which are conserved in the human and murine receptors. Tyr-457 and His-461 are essential for activity. Approximately 80% of the amino acids of the intracellular domain of the receptor is not required for major histocompatibility complex class I antigen induction or for antiviral protection against encephalomyocarditis virus. The observation that there was no protection by IFN-gamma against vesiculostomatitis virus indicates that other factors, in addition to chromosome 21 accessory factor(s), are required to generate the full complement of transduction signals from the human IFN-gamma receptor.

MeSH Terms
Amino Acid Sequence Animals CHO Cells Cricetinae Cytoplasm DNA Mutational Analysis Histocompatibility Antigens Class I/metabolism Humans Interferon-gamma/metabolism Molecular Sequence Data Receptors, Interferon/chemistry,physiology Recombinant Proteins/metabolism Structure-Activity Relationship Transfection Viral Interference
Chemicals
Histocompatibility Antigens Class I Receptors, Interferon Recombinant Proteins interferon gamma receptor Interferon-gamma
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cook J R
Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854.
Jung V
Schwartz B
Wang P
Pestka S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.23.11317
Published
1992-12-01
Pages
11317-21
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50541
Subset
IM
Grants
NCI NIH HHS · R01 CA46465 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.23.11317 PMC Full Text View journal details
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