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PMID: 6322158 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcripts of human HLA gene fragments lacking the 5'-terminal region in transfected mouse cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 3 ·1984-02-00 ·Pages 649-53

Yoshie O, Schmidt H, Lengyel P, Reddy ES, Morgan WR, Weissman SM

Abstract

Clones of mouse L cells transfected with a human HLA-B7 gene fragment lacking the 5' segment of exon 2 and all upstream sequences express HLA-specific transcripts of various lengths. These include species that correspond in size to full-length HLA-B7 mRNA. The level of these transcripts is increased in cells treated with interferon. It is probable that the full-length transcripts arise as a result of the linkage of the HLA-B7 gene fragments with DNA segments providing transcription initiation or polyadenylylation signals.

MeSH Terms

Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes Genes HLA Antigens/genetics Humans Major Histocompatibility Complex Mice Plasmids Transcription, Genetic Transfection

Chemicals

HLA Antigens DNA Restriction Enzymes

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Yoshie O

Schmidt H

Lengyel P

Reddy E S

Morgan W R

Weissman S M

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23 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.81.3.649

Published

1984-02-00

Pages

649-53

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC344892

Subset

Grants

NIAID NIH HHS · AI 12320 · United States

NCI NIH HHS · CA 16038 · United States

NCI NIH HHS · CA 30938 · United States

External Links

PubMed source DOI: 10.1073/pnas.81.3.649 PMC Full Text View journal details

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Transcripts of human HLA gene fragments lacking the 5'-terminal region in transfected mouse cells.

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