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PMID: 6959142 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

DNA sequence associated with chromosome translocations in mouse plasmacytomas.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 21 ·1982-11-00 ·Pages 6622-6

Harris LJ, D'Eustachio P, Ruddle FH, Marcu KB

Abstract

A DNA sequence that generates aberrantly rearranged immunoglobulin heavy chain constant region genes in murine plasmacytomas is shown to participate in a chromosome translocation. We have previously termed this DNA sequence NIARD for non-immunoglobulin-associated rearranging DNA. NIARD rearrangements were found frequently in murine plasmacytomas but were not detected in normal lymphocytes. These rearrangements occasionally involve the switch region of the C alpha gene. In this study, DNA samples obtained from mouse-Chinese hamster somatic cell hybrid lines were digested with various restriction endonucleases and analyzed by the Southern transfer technique with a NIARD hybridization probe. These experiments show that NIARD resides on chromosome 15 in the mouse germ line. Since NIARD is found adjacent to the C alpha gene (located on chromosome 12) in some plasmacytomas, it is apparent that a translocation involving these two chromosomes has occurred. We have proposed a rcpT(12;15) model to explain our data. The implications of NIARD rearrangements for malignant transformation are discussed.

MeSH Terms
Animals Chromosome Mapping Gene Expression Regulation Genes Immunoglobulins/genetics Mice Neoplasms, Experimental/genetics Plasmacytoma/genetics Translocation, Genetic
Chemicals
Immunoglobulins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Harris L J
D'Eustachio P
Ruddle F H
Marcu K B
References (42)
42 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.21.6622
Published
1982-11-00
Pages
6622-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347180
Subset
IM
Grants
NIAID NIH HHS · AI00416 · United States
NIGMS NIH HHS · GM09966 · United States
NIGMS NIH HHS · GM26939 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.21.6622 PMC Full Text View journal details
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