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PMID: 1454828 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Intramolecular DNA triplexes: unusual sequence requirements and influence on DNA polymerization.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 23 ·1992-12-01 ·Pages 11406-10

Dayn A, Samadashwily GM, Mirkin SM

Abstract

Homopurine-homopyrimidine mirror repeats are known to form intramolecular DNA triplexes in vitro. By probing with chemical agents specific for unusual DNA conformations, we have now demonstrated the formation of intramolecular triplexes consisting of G.G.C and T.A.T base triplets by DNA sequences that are neither homopurine-homopyrimidine nor mirror repeats. This finding significantly enlarges the number of sequences that could form DNA triplexes. The observed triplexes are stable under the conditions that are optimal for DNA polymerases in vitro. We found that triplex formation causes specific termination of DNA polymerization in vitro. This effect is detected for different DNA polymerases and may have implications for the regulation of DNA replication in vivo.

MeSH Terms
Base Sequence DNA/metabolism,ultrastructure DNA Replication DNA, Superhelical/metabolism DNA-Directed DNA Polymerase/metabolism Hydrogen Bonding In Vitro Techniques Molecular Sequence Data Nucleic Acid Conformation Structure-Activity Relationship Templates, Genetic
Chemicals
DNA, Superhelical DNA DNA-Directed DNA Polymerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dayn A
Department of Genetics, University of Illinois, Chicago 60612.
Samadashwily G M
Mirkin S M
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.23.11406
Published
1992-12-01
Pages
11406-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50559
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.23.11406 PMC Full Text View journal details
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