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PMID: 2377452 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA-sequence and metal-ion specificity of the formation of *H-DNA.

Nucleic acids research ·Vol. 18 ·No. 14 ·1990-07-25 ·Pages 4067-73

Bernués J, Beltrán R, Casasnovas JM, Azorín F

Abstract

The homopyrimidine-homopurine sequence d(CT/GA)22 undergoes, in the presence of zinc ions, transition to an altered DNA conformation (*H-DNA) which is neither H-DNA nor B-DNA. *H-DNA is characterized by a peculiar chemical reactivity pattern in which most of the polypyrimidine strand is hyperreactive to osmium tetroxide and the central part of the polypurine strand is sensitive to diethylpyrocarbonate. Formation of *H-DNA is specific of metal-ion. *H-DNA is detected in the presence of Zn++, Cd++ and Mn++. The efficiency on promoting the transition is in the order of Zn++ greater than Cd++ much greater than Mn++. Formation of *H-DNA is also specific of nucleotide sequence. From all the different homopolymeric sequences tested only the d(CT/GA)22 sequence showed the zinc-induced transition to *H-DNA. These results suggest that stabilization of *H-DNA involves the formation of a specific complex between the metal-ion and the nucleotide sequence. The biological relevance of these results is discussed in view of the important role that zinc ions play on many nucleic acids processes.

MeSH Terms
Base Sequence Cadmium Calcium Cobalt Copper DNA Magnesium Manganese Molecular Sequence Data Nickel Nucleic Acid Conformation Zinc
Chemicals
Cadmium Cobalt Manganese Copper Nickel DNA Magnesium Zinc Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bernués J
Grupo de Química Macromolecular, Centro de Investigación y Desarrollo CSIC, ETSEIB, Barcelona, Spain.
Beltrán R
Casasnovas J M
Azorín F
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.14.4067
Published
1990-07-25
Pages
4067-73
Language
English
Region
England
NLM ID
0411011
PMCID
PMC331161
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/18.14.4067 PMC Full Text View journal details
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