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PMID: 6329743 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The supercoil-stabilised cruciform of ColE1 is hyper-reactive to osmium tetroxide.

The EMBO journal ·Vol. 3 ·No. 5 ·1984-05-00 ·Pages 1187-92

Lilley DM, Palecek E

Abstract

Supercoiled pColIR215 contains a site of pronounced hyper-reactivity towards modification by osmium tetroxide, a reagent known to be single-strand-selective. The site of hypersensitivity has been mapped to the ColE1 inverted repeat, believed to extrude a cruciform in supercoiled DNA. Linear or relaxed plasmids are not modified by the reagent. We conclude that cruciform formation is responsible for the site-selective modification. Fine mapping of the modification site as a function of time has revealed that the initial reaction occurs at the centre of the inverted repeat, i.e., the unpaired loop of the cruciform, but that the modification region rapidly expands outwards from this point.

MeSH Terms
Bacteriocin Plasmids Binding Sites Chemical Phenomena Chemistry Colicins DNA Restriction Enzymes DNA, Superhelical Escherichia coli/genetics Nucleic Acid Conformation Osmium Osmium Tetroxide Plasmids
Chemicals
Colicins DNA, Superhelical Osmium DNA Restriction Enzymes Osmium Tetroxide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lilley D M
Palecek E
References (29)
29 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1984-05-00
Pages
1187-92
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557493
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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