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PMID: 2726457 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The propeller DNA conformation of poly(dA).poly(dT).

Nucleic acids research ·Vol. 17 ·No. 8 ·1989-04-25 ·Pages 3229-45

Aymami J, Coll M, Frederick CA, Wang AH, Rich A

Abstract

Physical properties of the DNA duplex, poly(dA).poly(dT) differ considerably from the alternating copolymer poly(dAT). A number of molecular models have been used to describe these structures obtained from fiber X-ray diffraction data. The recent solutions of single crystal DNA dodecamer structures with segments of oligo-A.oligo-T have revealed the presence of a high propeller twist in the AT regions which is stabilized by the formation of bifurcated (three-center) hydrogen bonds on the floor of the major groove, involving the N6 amino group of adenine hydrogen bonding to two O4 atoms of adjacent thymine residues on the opposite strand. Here we show that it is possible to incorporate the features of the single crystal analysis, specifically high propeller twist, bifurcated hydrogen bonds, and a narrow minor groove, as well as the close interstrand NMR signal between adenine HC2 and ribose HC1' of the opposite strand, into a model that is fully compatible with the diffraction data obtained from poly(dA).poly(dT).

MeSH Terms
Chemical Phenomena Chemistry, Physical Models, Molecular Nucleic Acid Conformation Poly dA-dT Polydeoxyribonucleotides X-Ray Diffraction
Chemicals
Polydeoxyribonucleotides Poly dA-dT
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Aymami J
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Coll M
Frederick C A
Wang A H
Rich A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.8.3229
Published
1989-04-25
Pages
3229-45
Language
English
Region
England
NLM ID
0411011
PMCID
PMC317725
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/17.8.3229 PMC Full Text View journal details
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