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PMID: 3627979 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Unique poly(dA).poly(dT) B'-conformation in cellular and synthetic DNAs.

Nucleic acids research ·Vol. 15 ·No. 15 ·1987-08-11 ·Pages 6063-74

Diekmann S, Zarling DA

Abstract

Poly(dA).poly(dT), but not B-form DNA, is specifically recognized by experimentally induced anti-kinetoplast or anti-poly(dA).poly(dT) immunoglobulins. Antibody binding is completely competed by poly(dA).poly(dT) and poly(dA).poly(dU) but not by other single- or double-stranded DNA sequences in a right-handed B-form. Antibody interaction with poly(dA).poly(dT) depends on immunoglobulin concentration, incubation time and temperature, and is sensitive to elevated ionic strengths. Similar conformations, for example, (dA)4-6 X (dT)4-6, in the kinetoplast DNA of the parasite Leishmania tarentolae are also immunogenic and induce specific anti-poly(dA).poly(dT) antibodies. These antibody probes specifically recognize nuclear and kinetoplast DNA in fixed flagellated kinetoplastid cells as evidenced by immunofluorescence microscopy. Anti-poly(dA).poly(dT) immunofluorescence is DNase-sensitive and competed by poly(dA).poly(dT), but not other classical double-stranded B-DNAs. Thus, these unique cellular B'-DNA helices are immunogenic and structurally similar to synthetic poly(dA).poly(dT) helices in solution.

MeSH Terms
Animals Antibody Specificity Female Immunoglobulins/immunology Nucleic Acid Conformation Poly dA-dT/immunology Polydeoxyribonucleotides/immunology Rabbits Sodium Chloride/pharmacology
Chemicals
Immunoglobulins Polydeoxyribonucleotides Poly dA-dT Sodium Chloride
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Diekmann S
Zarling D A
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.15.6063
Published
1987-08-11
Pages
6063-74
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306068
Subset
IM
Grants
NIGMS NIH HHS · GM-38424 · United States
External Links
PubMed source DOI: 10.1093/nar/15.15.6063 PMC Full Text View journal details
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