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PMID: 14582194 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Silkworm 5S RNA and alanine tRNA genes share highly conserved 5' flanking and coding sequences.

Molecular and cellular biology ·Vol. 2 ·No. 12 ·1982-12-00 ·Pages 1524-31

Morton DG, Sprague KU

Abstract

A fragment of Bombyx mori genomic DNA containing one tRNA2Ala gene and one 5S RNA gene has been used to compare the structural features of silkworm 5S RNA and tRNA genes. The nucleotide sequences of both genes and of the primary transcripts produced from them in homologous in vitro transcription systems have been determined. Comparison of the sequences of these two genes with that of another previously analyzed B. mori tRNA2Ala gene reveals common oligonucleotides which may be important transcriptional signals. The oligonucleotides TA(C)TAT, AATTTT, and TTC are located approximately (+/- 1 nucleotide) 29, 19, and 3 nucleotides, respectively, before the transcription initiation sites of the two tRNA2Ala genes and the one 5S RNA gene we have analyzed. The sequence GGGCGTAG(C)TCAG lies within the coding regions of all three genes. The functional significance of these sequences is suggested by their location within regions required for the transcription of silkworm alanine tRNA genes in vitro.

MeSH Terms
5' Flanking Region/genetics Animals Base Sequence Bombyx/genetics Genes, Insect Molecular Sequence Data RNA/genetics RNA, Transfer, Amino Acyl/genetics Transcription, Genetic
Chemicals
RNA, Transfer, Amino Acyl RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Morton D G
Institute of Molecular Biology and Biology Department, University of Oregon, Eugene, Oregon 97403, USA.
Sprague K U
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.2.12.1524-1531.1982
Published
1982-12-00
Pages
1524-31
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369961
Subset
IM
Grants
NIGMS NIH HHS · GM 25388 · United States
NICHD NIH HHS · HD00420 · United States
External Links
PubMed source DOI: 10.1128/mcb.2.12.1524-1531.1982 PMC Full Text View journal details
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