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PMID: 14610055 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A core function for p120-catenin in cadherin turnover.

The Journal of cell biology ·Vol. 163 ·No. 3 ·2003-11-10 ·Pages 525-34

Davis MA, Ireton RC, Reynolds AB

Abstract

p120-catenin stabilizes epithelial cadherin (E-cadherin) in SW48 cells, but the mechanism has not been established. Here, we show that p120 acts at the cell surface to control cadherin turnover, thereby regulating cadherin levels. p120 knockdown by siRNA expression resulted in dose-dependent elimination of epithelial, placental, neuronal, and vascular endothelial cadherins, and complete loss of cell-cell adhesion. ARVCF and delta-catenin were functionally redundant, suggesting that proper cadherin-dependent adhesion requires the presence of at least one p120 family member. The data reveal a core function of p120 in cadherin complexes, and strongly predict a dose-dependent loss of E-cadherin in tumors that partially or completely down-regulate p120.

MeSH Terms
Adherens Junctions/genetics,metabolism Armadillo Domain Proteins Cadherins/genetics,metabolism Catenins Cell Adhesion/physiology Cell Adhesion Molecules/antagonists & inhibitors,genetics,metabolism Cell Line, Tumor Cell Membrane/metabolism Cell Transformation, Neoplastic/genetics,metabolism Cytoskeletal Proteins/genetics,metabolism Down-Regulation/genetics Humans Models, Biological Neoplasms/genetics,metabolism Phosphoproteins/antagonists & inhibitors,genetics,metabolism RNA, Small Interfering/pharmacology
Chemicals
ARVCF protein, human Armadillo Domain Proteins Cadherins Catenins Cell Adhesion Molecules Cytoskeletal Proteins Phosphoproteins RNA, Small Interfering delta catenin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Davis Michael A
Department of Cancer Biology, Vanderbilt University, Nashville, TN 37232-6840, USA.
Ireton Renee C
Reynolds Albert B
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200307111
Published
2003-11-10
Pages
525-34
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2173649
Subset
IM
Grants
NCI NIH HHS · P50 CA095103 · United States
NCI NIH HHS · 1P50 CA95103 · United States
NCI NIH HHS · CA69485 · United States
NCI NIH HHS · CA55724 · United States
NCI NIH HHS · R01 CA055724 · United States
Corrections
CommentIn
PMID: 14610049
External Links
PubMed source DOI: 10.1083/jcb.200307111 PMC Full Text View journal details
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