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PMID: 146192 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of Neurospora crassa cytoplasmic initiator tRNA.

Nucleic acids research ·Vol. 4 ·No. 12 ·1977-12-00 ·Pages 4109-31

Gillum AM, Hecker LI, Silberklang M, Schwartzbach SD, RajBhandary UL, Barnett WE

Abstract

Initiator methionine tRNA from the cytoplasm of Neurospora crassa has been purified and sequenced. The sequence is: pAGCUGCAUm1GGCGCAGCGGAAGCGCM22GCY*GGGCUCAUt6AACCCGGAGm7GU (or D) - CACUCGAUCGm1AAACGAG*UUGCAGCUACCAOH. Similar to initiator tRNAs from the cytoplasm of other eukaryotes, this tRNA also contains the sequence -AUCG- instead of the usual -TphiCG (or A)- found in loop IV of other tRNAs. The sequence of the N. crassa cytoplasmic initiator tRNA is quite different from that of the corresponding mitochondrial initiator tRNA. Comparison of the sequence of N. crassa cytoplasmic initiator tRNA to those of yeast, wheat germ and vertebrate cytoplasmic initiator tRNA indicates that the sequences of the two fungal tRNAs are no more similar to each other than they are to those of other initiator tRNAs.

MeSH Terms
Amino Acyl-tRNA Synthetases Base Sequence Methionine Neurospora/analysis Neurospora crassa/analysis Oligoribonucleotides/analysis Peptide Chain Initiation, Translational RNA, Transfer Ribonucleases
Chemicals
Oligoribonucleotides RNA, Transfer Methionine Ribonucleases Amino Acyl-tRNA Synthetases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Gillum A M
Hecker L I
Silberklang M
Schwartzbach S D
RajBhandary U L
Barnett W E
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36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.12.4109
Published
1977-12-00
Pages
4109-31
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343229
Subset
IM
Databases
GENBANK
K00316
External Links
PubMed source DOI: 10.1093/nar/4.12.4109 PMC Full Text View journal details
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