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PMID: 14630966 Published · ppublish English Journal Article

Engineering vitamin E content: from Arabidopsis mutant to soy oil.

The Plant cell ·Vol. 15 ·No. 12 ·2003-12-00 ·Pages 3007-19

Van Eenennaam AL, Lincoln K, Durrett TP, Valentin HE, Shewmaker CK, Thorne GM, Jiang J, Baszis SR, Levering CK, Aasen ED, Hao M, Stein JC, Norris SR, Last RL

Abstract

We report the identification and biotechnological utility of a plant gene encoding the tocopherol (vitamin E) biosynthetic enzyme 2-methyl-6-phytylbenzoquinol methyltransferase. This gene was identified by map-based cloning of the Arabidopsis mutation vitamin E pathway gene3-1 (vte3-1), which causes increased accumulation of delta-tocopherol and decreased gamma-tocopherol in the seed. Enzyme assays of recombinant protein supported the hypothesis that At-VTE3 encodes a 2-methyl-6-phytylbenzoquinol methyltransferase. Seed-specific expression of At-VTE3 in transgenic soybean reduced seed delta-tocopherol from 20 to 2%. These results confirm that At-VTE3 protein catalyzes the methylation of 2-methyl-6-phytylbenzoquinol in planta and show the utility of this gene in altering soybean tocopherol composition. When At-VTE3 was coexpressed with At-VTE4 (gamma-tocopherol methyltransferase) in soybean, the seed accumulated to >95% alpha-tocopherol, a dramatic change from the normal 10%, resulting in a greater than eightfold increase of alpha-tocopherol and an up to fivefold increase in seed vitamin E activity. These findings demonstrate the utility of a gene identified in Arabidopsis to alter the tocopherol composition of commercial seed oils, a result with both nutritional and food quality implications.

MeSH Terms
Alleles Amino Acid Sequence Arabidopsis/chemistry,genetics,metabolism Arabidopsis Proteins/genetics,metabolism Cloning, Molecular DNA, Complementary/chemistry,genetics Escherichia coli/genetics,metabolism Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Methyltransferases/genetics,metabolism Molecular Sequence Data Mutation Plants, Genetically Modified Sequence Analysis, DNA Sequence Homology, Amino Acid Soybean Oil/metabolism Soybeans/enzymology,genetics Tocopherols/metabolism Vitamin E/biosynthesis
Chemicals
Arabidopsis Proteins DNA, Complementary Vitamin E Soybean Oil 2-methyl-6-phytylbenzoquinol methyltransferase, Arabidopsis 2-methyl-6-phytylbenzoquinone methyltransferase Methyltransferases delta-tocopherol Tocopherols
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Van Eenennaam Alison L
Monsanto Company, Calgene Campus, Davis, California 95616, USA.
Lincoln Kim
Durrett Timothy P
Valentin Henry E
Shewmaker Christine K
Thorne Greg M
Jiang Jian
Baszis Susan R
Levering Charlene K
Aasen Eric D
Hao Ming
Stein Joshua C
Norris Susan R
Last Robert L
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
Published
2003-12-00
Epub
2003-00-20
Pages
3007-19
Language
English
Region
England
NLM ID
9208688
PMCID
PMC282849
Subset
IM
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