Abstract
A study of the biochemical genetics of the Arabidopsis thaliana tryptophan synthase beta subunit was initiated by characterization of mutants resistant to the inhibitor 5-fluoroindole. Thirteen recessive mutations were recovered that are allelic to trp2-1, a mutation in the more highly expressed of duplicate tryptophan synthase beta subunit genes (TSB1). Ten of these mutations (trp2-2 through trp2-11) cause a tryptophan requirement (auxotrophs), whereas three (trp2-100 through trp2-102) remain tryptophan prototrophs. The mutations cause a variety of changes in tryptophan synthase beta expression. For example, two mutations (trp2-5 and trp2-8) cause dramatically reduced accumulation of TSB mRNA and immunologically detectable protein, whereas trp2-10 is associated with increased mRNA and protein. A correlation exists between the quantity of mutant beta and wild-type alpha subunit levels in the trp2 mutant plants, suggesting that the synthesis of these proteins is coordinated or that the quantity or structure of the beta subunit influences the stability of the alpha protein. The level of immunologically detectable anthranilate synthase alpha subunit protein is increased in the trp2 mutants, suggesting the possibility of regulation of anthranilate synthase levels in response to tryptophan limitation.
MeSH Terms
Alleles
Amino Acid Sequence
Arabidopsis/drug effects,enzymology,genetics
Base Sequence
Drug Resistance/genetics
Genes, Plant
Indoles/pharmacology
Molecular Sequence Data
Mutagenesis
Plant Proteins/biosynthesis,genetics,physiology
Tryptophan Synthase/biosynthesis,genetics,physiology
Chemicals
Indoles
Plant Proteins
5-fluoroindole
Tryptophan Synthase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Barczak A J
Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York 14853-1801, USA.
Zhao J
Pruitt K D
Last R L
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