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PMID: 14668396 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

An analysis of microsatellite loci in Arabidopsis thaliana: mutational dynamics and application.

Genetics ·Vol. 165 ·No. 3 ·2003-11-00 ·Pages 1475-88

Symonds VV, Lloyd AM

Abstract

Microsatellite loci are among the most commonly used molecular markers. These loci typically exhibit variation for allele frequency distribution within a species. However, the factors contributing to this variation are not well understood. To expand on the current knowledge of microsatellite evolution, 20 microsatellite loci were examined for 126 accessions of the flowering plant, Arabidopsis thaliana. Substantial variability in mutation pattern among loci was found, most of which cannot be explained by the assumptions of the traditional stepwise mutation model or infinite alleles model. Here it is shown that the degree of locus diversity is strongly correlated with the number of contiguous repeats, more so than with the total number of repeats. These findings support a strong role for repeat disruptions in stabilizing microsatellite loci by reducing the substrate for polymerase slippage and recombination. Results of cluster analyses are also presented, demonstrating the potential of microsatellite loci for resolving relationships among accessions of A. thaliana.

MeSH Terms
Arabidopsis/genetics Base Sequence Cloning, Molecular DNA, Plant Evolution, Molecular Microsatellite Repeats/genetics Molecular Sequence Data Mutation Sequence Homology, Amino Acid
Chemicals
DNA, Plant
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Symonds V Vaughan
Section of Molecular, Cell, and Developmental Biology and Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA.
Lloyd Alan M
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/165.3.1475
Published
2003-11-00
Pages
1475-88
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1462854
Subset
IM
Databases
GENBANK
AY293992, AY293993, AY293994, AY293995, AY293996, AY293997, AY293998, AY293999, AY294000, AY294001, AY294002, AY294003, AY294004, AY295838, AY295839, AY295840, AY295841, AY295842, AY295843, AY295844, AY295845, AY295846, AY295847, AY295848, AY295849, AY295850, AY295851, AY295852, AY295853, AY295854, AY295855, AY295856, AY295857, AY295858, AY295859, AY295860, AY295861, AY295862, AY295863, AY295864, AY295865, AY295866, AY295867, AY295868, AY295869, AY295870, AY295871
External Links
PubMed source DOI: 10.1093/genetics/165.3.1475 PMC Full Text View journal details
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