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PMID: 14704204 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Fission yeast Mus81.Eme1 Holliday junction resolvase is required for meiotic crossing over but not for gene conversion.

Genetics ·Vol. 165 ·No. 4 ·2003-12-00 ·Pages 2289-93

Smith GR, Boddy MN, Shanahan P, Russell P

Abstract

Most models of homologous recombination invoke cleavage of Holliday junctions to explain crossing over. The Mus81.Eme1 endonuclease from fission yeast and humans cleaves Holliday junctions and other branched DNA structures, leaving its physiological substrate uncertain. We report here that Schizosaccharomyces pombe mus81 mutants have normal or elevated frequencies of gene conversion but 20- to 100-fold reduced frequencies of crossing over. Thus, gene conversion and crossing over can be genetically separated, and Mus81 is required for crossing over, supporting the hypothesis that the fission yeast Mus81.Eme1 protein complex resolves Holliday junctions in meiotic cells.

MeSH Terms
Crossing Over, Genetic DNA Damage DNA-Binding Proteins/physiology Endonucleases/physiology Gene Conversion Meiosis/physiology Saccharomyces cerevisiae Proteins Schizosaccharomyces/enzymology,genetics,radiation effects Schizosaccharomyces pombe Proteins/physiology
Chemicals
DNA-Binding Proteins Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Eme1 protein, S pombe Endonucleases MUS81 protein, S cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Smith Gerald R
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA. [email protected]
Boddy Michael N
Shanahan Paul
Russell Paul
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/165.4.2289
Email
[email protected]
Published
2003-12-00
Pages
2289-93
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1462924
Subset
IM
Grants
NIGMS NIH HHS · GM-32194 · United States
NIGMS NIH HHS · GM-59447 · United States
External Links
PubMed source DOI: 10.1093/genetics/165.4.2289 PMC Full Text View journal details
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