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PMID: 14749723 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DJ-1 has a role in antioxidative stress to prevent cell death.

EMBO reports ·Vol. 5 ·No. 2 ·2004-02-00 ·Pages 213-8

Taira T, Saito Y, Niki T, Iguchi-Ariga SM, Takahashi K, Ariga H

Abstract

Deletion and point (L166P) mutations of DJ-1 have recently been shown to be responsible for the onset of familial Parkinson's disease (PD, PARK7). The aim of this study was to determine the role of DJ-1 in PD. We first found that DJ-1 eliminated hydrogen peroxide in vitro by oxidizing itself. We then found that DJ-1 knockdown by short interfering RNA rendered SH-SY5Y neuroblastoma cells susceptible to hydrogen peroxide-, MPP+- or 6-hydroxydopamine-induced cell death and that cells harbouring mutant forms of DJ-1, including L166P, became susceptible to death in parallel with the loss of oxidized forms of DJ-1. These results clearly showed that DJ-1 has a role in the antioxidative stress reaction and that mutations of DJ-1 lead to cell death, which is observed in PD.

MeSH Terms
Animals Cell Death Cell Line Cell Survival/drug effects Gene Expression Humans Hydrogen Peroxide/metabolism,pharmacology Intracellular Signaling Peptides and Proteins Mice Mutation/genetics NIH 3T3 Cells Oncogene Proteins/genetics,physiology Oxidative Stress Parkinson Disease/genetics,metabolism Protein Deglycase DJ-1 RNA Interference RNA, Small Interfering/pharmacology
Chemicals
Intracellular Signaling Peptides and Proteins Oncogene Proteins RNA, Small Interfering Hydrogen Peroxide PARK7 protein, human Protein Deglycase DJ-1
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Taira Takahiro
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Sapporo 060-0812, Japan.
Saito Yoshiro
Niki Takeshi
Iguchi-Ariga Sanae M M
Takahashi Kazuhiko
Ariga Hiroyoshi
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Article Info
Journal
EMBO reports
Abbr.
EMBO Rep
ISSN
1469-221X
DOI
10.1038/sj.embor.7400074
Published
2004-02-00
Epub
2004-00-23
Pages
213-8
Language
English
Region
England
NLM ID
100963049
PMCID
PMC1298985
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1038/sj.embor.7400074 PMC Full Text View journal details
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