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PMID: 1475192 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

RNA-primed complementary-sense DNA synthesis of the geminivirus African cassava mosaic virus.

Nucleic acids research ·Vol. 20 ·No. 23 ·1992-12-11 ·Pages 6311-5

Saunders K, Lucy A, Stanley J

Abstract

The plant DNA virus African cassava mosaic virus (ACMV) is believed to replicate by a rolling circle mechanism. To investigate complementary-sense DNA (lagging strand) synthesis, we have analysed the heterogenous form of complementary-sense DNA (H3 DNA) from infected Nicotiana benthamiana by two-dimensional agarose gel electrophoresis and blot hybridisation. The presence of an RNA moeity is demonstrated by comparison of results for nucleic acids resolved on neutral/alkaline and neutral/formamide gels, suggesting that complementary-sense DNA synthesis on the virus-sense single-stranded DNA template is preceded by the synthesis of an RNA primer. Hybridisation with probes to specific parts of ACMV DNA A genome indicates that synthesis of the putative RNA primer initiates between nucleotides 2581-221, a region that includes intergenic sequences that have been implicated in geminivirus DNA replication and the control of gene expression.

MeSH Terms
DNA, Viral/biosynthesis Electrophoresis, Agar Gel Electrophoresis, Gel, Two-Dimensional Formamides Mosaic Viruses/genetics Plants, Toxic RNA, Viral/metabolism Tobacco
Chemicals
DNA, Viral Formamides RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Saunders K
Department of Virus Research, John Innes Institute, John Innes Centre for Plant Science Research, Norwich, UK.
Lucy A
Stanley J
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21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.23.6311
Published
1992-12-11
Pages
6311-5
Language
English
Region
England
NLM ID
0411011
PMCID
PMC334521
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/20.23.6311 PMC Full Text View journal details
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