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PMID: 3022243 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular characterisation of subgenomic single-stranded and double-stranded DNA forms isolated from plants infected with tomato golden mosaic virus.

Nucleic acids research ·Vol. 14 ·No. 20 ·1986-10-24 ·Pages 7967-84

MacDowell SW, Coutts RH, Buck KW

Abstract

A subgenomic single-stranded DNA present in particles of the geminivirus, tomato golden mosaic virus, has been shown by electron microscope heteroduplex mapping and Southern hybridisation analysis to consist of circular molecules, ca. 1.2 kb in size, derived from the smaller of the two genomic DNA components, DNA B, by deletion of open reading frame (ORF) BR1 and the C-terminal portion of ORF BL1. A covalently closed circular, supercoiled, double-stranded form of the subgenomic DNA has been isolated from virus-infected plants and cloned into pEMBL9. Analysis of the sequence of 22 clones across the deletion boundaries revealed only four different deletion boundaries, derived from four different left hand borders and three different right hand borders. Each border was within a region of 11 nucleotides and gave rise to a narrow size range (1248-1261 nucleotides) for the population of 22 subgenomic DNAs. However apparently smaller subgenomic DNAs were sometimes formed when plants were inoculated with cloned subgenomic DNA, or a construct derived from a subgenomic DNA in which a neomycin phosphotransferase gene had been inserted, together with the genomic DNA components. Mechanisms to account for the size, specificity and formation of the subgenomic DNA are discussed.

MeSH Terms
Base Sequence Chromosome Mapping Cloning, Molecular DNA/genetics DNA Restriction Enzymes DNA, Circular/genetics DNA, Single-Stranded/genetics DNA, Viral/genetics Mosaic Viruses/genetics,pathogenicity Mutation Nucleic Acid Hybridization Plants/microbiology
Chemicals
DNA, Circular DNA, Single-Stranded DNA, Viral DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
MacDowell S W
Coutts R H
Buck K W
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32 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.20.7967
Published
1986-10-24
Pages
7967-84
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311828
Subset
IM
Databases
GENBANK
X04485
External Links
PubMed source DOI: 10.1093/nar/14.20.7967 PMC Full Text View journal details
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