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PMID: 6326051 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence preference at rat liver and wheat germ type 1 DNA topoisomerase breakage sites in duplex SV40 DNA.

Nucleic acids research ·Vol. 12 ·No. 7 ·1984-04-11 ·Pages 3097-114

Been MD, Burgess RR, Champoux JJ

Abstract

The site specificities of the type 1 DNA topoisomerases (topo 1) from rat liver and wheat germ were investigated. The nucleotide sequence at break sites on duplex SV40 DNA were determined for 245 wheat germ topo 1 sites and 223 rat liver topo 1 sites over a region of 1781 nucleotides. The enzymes from the two different sources show similar, but not identical patterns of DNA strand breakage. The sites occur frequently, but are not broken with equal probabilities. Major sites of breakage occur on the average every one to two turns of the helix, thus if sites of breakage accurately represent topo 1 sites of activity, the DNA sequence alone would appear to place few limits on the access of the enzyme to DNA. Sequences around the strongest sites for both enzymes show a bias in base composition for the four nucleotides immediately 5' to the break site (-4 to -1 positions), but no bias is observed 3' to the site of breakage. Consensus sequences for both enzymes were determined. Variations from the consensus sequence appear to affect the two enzymes differently and may account for the differences observed in the specificity of breakage.

MeSH Terms
Animals Base Sequence DNA Topoisomerases, Type I/metabolism DNA, Viral/metabolism Liver/enzymology Plants/enzymology Rats Simian virus 40 Substrate Specificity Triticum/enzymology
Chemicals
DNA, Viral DNA Topoisomerases, Type I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Been M D
Burgess R R
Champoux J J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.7.3097
Published
1984-04-11
Pages
3097-114
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318732
Subset
IM
Grants
NCI NIH HHS · CA07175 · United States
NCI NIH HHS · CA23076 · United States
NIGMS NIH HHS · GM23224 · United States
External Links
PubMed source DOI: 10.1093/nar/12.7.3097 PMC Full Text View journal details
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