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PMID: 14769858 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Capping protein binding to actin in yeast: biochemical mechanism and physiological relevance.

The Journal of cell biology ·Vol. 164 ·No. 4 ·2004-02-16 ·Pages 567-80

Kim K, Yamashita A, Wear MA, Maéda Y, Cooper JA

Abstract

The mechanism by which capping protein (CP) binds barbed ends of actin filaments is not understood, and the physiological significance of CP binding to actin is not defined. The CP crystal structure suggests that the COOH-terminal regions of the CP alpha and beta subunits bind to the barbed end. Using purified recombinant mutant yeast CP, we tested this model. CP lacking both COOH-terminal regions did not bind actin. The alpha COOH-terminal region was more important than that of beta. The significance of CP's actin-binding activity in vivo was tested by determining how well CP actin-binding mutants rescued null mutant phenotypes. Rescue correlated well with capping activity, as did localization of CP to actin patches, indicating that capping is a physiological function for CP. Actin filaments of patches appear to be nucleated first, then capped with CP. The binding constants of yeast CP for actin suggest that actin capping in yeast is more dynamic than in vertebrates.

MeSH Terms
Actin Capping Proteins Actin Depolymerizing Factors Actins/metabolism Amino Acid Sequence Animals Chickens Cytoskeletal Proteins Destrin Humans Microfilament Proteins/chemistry,genetics,metabolism Models, Molecular Molecular Sequence Data Point Mutation Protein Binding Protein Structure, Tertiary Proteins/chemistry,genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/chemistry,genetics,metabolism Sequence Alignment
Chemicals
Actin Capping Proteins Actin Depolymerizing Factors Actins CAP1 protein, S cerevisiae Cap2 protein, S cerevisiae Cytoskeletal Proteins Destrin Microfilament Proteins Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kim Kyoungtae
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Yamashita Atsuko
Wear Martin A
Maéda Yuichiro
Cooper John A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200308061
Published
2004-02-16
Epub
2004-00-09
Pages
567-80
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2171992
Subset
IM
Grants
NIGMS NIH HHS · R01 GM038542 · United States
NIGMS NIH HHS · R01 GM038542-15 · United States
NIGMS NIH HHS · R01 GM047337 · United States
NIGMS NIH HHS · GM47337 · United States
External Links
PubMed source DOI: 10.1083/jcb.200308061 PMC Full Text View journal details
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