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PMID: 1487827 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The role of anticodon bases and the discriminator nucleotide in the recognition of some E. coli tRNAs by their aminoacyl-tRNA synthetases.

Journal of molecular evolution ·Vol. 35 ·No. 5 ·1992-11-00 ·Pages 436-43

Shimizu M, Asahara H, Tamura K, Hasegawa T, Himeno H

Abstract

The T7 polymerase transcription system was used for in vitro synthesis of unmodified versions of the E. coli tRNA mutants that insert asparagine, cysteine, glycine, histidine, and serine. These tRNAs were used to qualitatively explore the role of some anticodon bases and the discriminator nucleotide in the recognition of tRNA by aminoacyl-tRNA synthetases. Coupled with data from earlier studies, these new results essentially complete a survey of all E. coli tRNAs with respect to the involvement of anticodon bases and the discriminator nucleotide in tRNA recognition. It is found that in the vast majority of tRNAs both of these elements are significant components of tRNA identity. This is not universally true, however. Anticodon sequences are unimportant in tRNA(Ser), tRNA(Leu), and tRNA(Ala) while the discriminator base is inconsequential in tRNA(Ser) and tRNA(Thr). The significance of these results for origin-of-life studies is discussed.

MeSH Terms
Amino Acyl-tRNA Synthetases/metabolism Anticodon/genetics Asparagine/metabolism Bacteriophage T7 Base Sequence Cysteine/metabolism DNA-Directed RNA Polymerases Escherichia coli/genetics,metabolism Glycine/metabolism Histidine/metabolism Molecular Sequence Data Nucleic Acid Conformation RNA, Transfer/genetics,metabolism Serine/metabolism Structure-Activity Relationship Substrate Specificity
Chemicals
Anticodon Serine Histidine Asparagine RNA, Transfer DNA-Directed RNA Polymerases Amino Acyl-tRNA Synthetases Cysteine Glycine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Shimizu M
Institute of Space and Astronautical Science, Kanagawa Pref., Japan.
Asahara H
Tamura K
Hasegawa T
Himeno H
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Article Info
Journal
Journal of molecular evolution
Abbr.
J Mol Evol
ISSN
0022-2844
DOI
10.1007/BF00171822
Published
1992-11-00
Pages
436-43
Language
English
Region
Germany
NLM ID
0360051
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1007/BF00171822 View journal details
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